rmbiomed
A Standardized Inverter Package deal Borne by Broad Host Vary Plasmids for Genetic Circuit Design in Gram-Damaging Micro organism
Genetically encoded logic gates, particularly inverters-NOT gates-are the constructing blocks for designing circuits, engineering biosensors, or decision-making gadgets in artificial biology. Nonetheless, the repertoire of inverters available for various species is moderately restricted. On this work, a big complete of NOT gates that was proven to operate beforehand in a particular pressure of Escherichia coli, was recreated as broad host vary (BHR) assortment of constructs assembled in low, medium, and excessive copy quantity plasmid backbones of the SEVA (Normal European Vector Structure) assortment.
The enter/output operate of every of the gates was characterised and parametrized within the environmental bacterium and metabolic engineering chassis Pseudomonas putida. Comparisons of the ensuing fluorescence cytometry information with these printed for a similar gates in Escherichia coli supplied helpful hints on the portability of the corresponding gates.
The hereby described inverter bundle (20 totally different variations of 12 distinct gates) borne by BHR plasmids thus turns into a toolbox of selection for designing genetic circuitries in quite a lot of Gram-negative species apart from E. coli.
Formulation and characterization of nanoemulsion from Alhagi maurorum important oil and research of its antimicrobial, antibiofilm, and plasmid curing exercise in opposition to antibiotic-resistant pathogenic micro organism
Nanoemulsion expertise is an alternate candidate to beat antibiotic resistance in pathogenic micro organism. The intention of this analysis was nanoemulsion manufacturing from the important oil of Alhagi maurorum and the characterization of this nanostructure. Nanoemulsion of important oil from A. maurorum was ready utilizing the ionotropic gelation technique and chitosan as a nano-carrier. Scanning Electron Microscopy (SEM) was used to characterize the synthesized nanoparticles.
The impact of nanoemulsion on the antibacterial, antibiofilm, and plasmid curing of six antibiotic-resistant pathogenic micro organism (P. aeruginosa, E. coli, S. aureus, Okay. pneumonia, A. baumannii, B. cereus) was evaluated.
- The outcomes of this research confirmed that nanoparticles had a spherical form and easy topology. The imply dimension had been 172 ± four nm and Zeta potentials was +28.6 mv. The outcomes of antibacterial exercise confirmed that nanoemulsion of important oil had larger inhibition in opposition to micro organism in comparison with free important oil.
- Additionally, this nanoemulsion had antibiofilm exercise. Minimal Inhibitory Focus (MIC) and Minimal Bactericidal Focus for Biofilm (MBCB) had been decided for nanoemulsion in opposition to the biofilm of pathogenic micro organism.
- The outcomes have proven that the MIC worth for A. baumannii is 12.5 mg ml -1 and for E. coli this worth is 1.75 mg ml -1. This discovering implies that MIC values had been highest for A. baumannii and lowest for E. coli. Statistical evaluation demonstrated that the inhibitory impact of nanoemulsion in opposition to bacterial biofilm was important (P < 0.05).
- This nanoemulsion additionally had a exceptional impact the curing of R-plasmid of three antibiotic-resistant micro organism. Based on GC-MS evaluation of A. maurorum important oil, the principle compounds had been oxygenated sesquiterpenes and hydrocarbons. Nanoemulsion of A. maurorum had the potential to make use of as appropriate antimicrobial brokers in opposition to antibiotic-resistant micro organism.
Emergence of plasmid-mediated mcr genes from Gram-negative micro organism on the human-animal interface
Background: The worldwide emergence of plasmid-mediated colistin resistance (Col-R) conferred by mcr genes in gram-negative rods (GNRs) has jeopardized the final therapy possibility for multidrug-resistant bacterial infections in people. This research aimed to evaluate the emergence of mcr gene-mediated Col-R in GNRs remoted from people and animals in Pakistan.
Strategies: Animal and medical specimens collected from numerous sources had been prospectively analysed utilizing commonplace microbiological procedures. Pathogens had been recognized utilizing the API 20E and API 20NE methods (bioMerieux).
Minimal inhibitory focus (MIC) in opposition to colistin was decided utilizing the MIC detection strategies, and multiplex polymerase chain response (PCR) was used to amplify the mcr-1 to mcr-5 genes.
Outcomes: We remoted 126 (88.1%) animal and 17 (11.9%) human Col-R phenotypes, amongst which there was a big affiliation (P < 0.01) of Escherichia coli and Proteus mirabilis with animals and of Acinetobacter baumannii with people.
Animal strains exhibited statistically important (P < 0.05) resistance to co-trimoxazole, chloramphenicol, and moxifloxacin, and the human pathogens exhibited statistically important (P < 0.05) antibiotic resistance to cephalosporins, carbapenems, and piperacillin-tazobactam.
For Col-R strains, MIC50 values had been > 6 µg/mL and > 12 µg/mL for human and animal isolates, respectively. mcr genes had been detected in 110 (76.9%) bacterial strains, of which 108 (98.2%) had been mcr-1 and a couple of (1.8%) had been mcr-2.
Conclusions: The detection of a substantial variety of MCR-1 and MCR-2 genes in animals is worrisome, as they’re now being detected in medical pathogens. The acquisition of mcr genes by colistin-susceptible micro organism might go away us in a post-antibiotic period.
Key phrases: Antibacterial profile; Antimicrobial resistance; Colistin resistance; Cell colistin resistance; Plasmid-mediated resistance; mcr genes.
Recombinant Bacillus anthracis Plasmid replication protein RepX (repX) |
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| MBS1414127-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1305 |
Recombinant Bacillus anthracis Plasmid replication protein RepX (repX) |
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| MBS1414127-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 990 |
Recombinant Bacillus anthracis Plasmid replication protein RepX (repX) |
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| MBS1414127-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Bacillus anthracis Plasmid replication protein RepX (repX) |
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| MBS1414127-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1155 |
Recombinant Bacillus anthracis Plasmid replication protein RepX (repX) |
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| MBS1414127-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) |
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| MBS6013258-02mL | MyBiosource | 0.2(mL | EUR 695 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) |
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| MBS6013258-5x02mL | MyBiosource | 5x0.2mL | EUR 2975 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) |
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| MBS626778-01mg | MyBiosource | 0.1mg | EUR 740 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) |
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| MBS626778-5x01mg | MyBiosource | 5x0.1mg | EUR 3175 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) |
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| MBS620291-01mg | MyBiosource | 0.1mg | EUR 905 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) |
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| MBS620291-5x01mg | MyBiosource | 5x0.1mg | EUR 3925 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (PE) |
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| MBS6498208-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (PE) |
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| MBS6498208-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (AP) |
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| MBS6498198-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (AP) |
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| MBS6498198-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (APC) |
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| MBS6498199-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (APC) |
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| MBS6498199-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (Biotin) |
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| MBS6498200-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (Biotin) |
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| MBS6498200-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (FITC) |
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| MBS6498201-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (FITC) |
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| MBS6498201-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (HRP) |
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| MBS6498202-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (HRP) |
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| MBS6498202-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (AP) |
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| MBS6343171-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (AP) |
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| MBS6343171-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (APC) |
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| MBS6343172-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (APC) |
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| MBS6343172-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (FITC) |
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| MBS6343174-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (FITC) |
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| MBS6343174-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (HRP) |
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| MBS6343175-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (HRP) |
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| MBS6343175-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (PE) |
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| MBS6343181-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (PE) |
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| MBS6343181-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 490) |
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| MBS6498204-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 550) |
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| MBS6498205-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 550) |
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| MBS6498205-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 650) |
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| MBS6498206-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 650) |
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| MBS6498206-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 750) |
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| MBS6498207-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 750) |
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| MBS6498207-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 405) |
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| MBS6498203-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 405) |
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| MBS6498203-5x01mL | MyBiosource | 5x0.1mL | EUR 4310 |
RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) (MaxLight 490) |
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| MBS6498204-01mL | MyBiosource | 0.1mL | EUR 990 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (Biotin) |
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| MBS6343173-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (Biotin) |
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| MBS6343173-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 405) |
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| MBS6343176-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 405) |
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| MBS6343176-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 490) |
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| MBS6343177-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 490) |
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| MBS6343177-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 550) |
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| MBS6343178-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 550) |
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| MBS6343178-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 650) |
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| MBS6343179-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 650) |
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| MBS6343179-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 750) |
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| MBS6343180-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA2, NT (RPA2, REPA2, RPA32, RPA34, Replication protein A 32kD subunit, Replication factor A protein 2, Replication protein A 34kD subunit) (MaxLight 750) |
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| MBS6343180-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) |
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| MBS646008-02mL | MyBiosource | 0.2mL | EUR 695 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) |
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| MBS646008-5x02mL | MyBiosource | 5x0.2mL | EUR 2975 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (AP) |
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| MBS6343182-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (AP) |
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| MBS6343182-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (PE) |
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| MBS6343192-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (PE) |
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| MBS6343192-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
Replication Protein A2 (RPA2) |
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| MBS4380680-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
Replication Protein A2 (RPA2) |
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| MBS4380680-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
Replication Protein A2 (RPA2) |
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| MBS4380680-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
Replication Protein A2 (RPA2) |
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| MBS4380680-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
Replication Protein A2 (RPA2) |
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| MBS4380680-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
Replication Protein A2 (RPA2) |
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| MBS4381233-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
Replication Protein A2 (RPA2) |
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| MBS4381233-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
Replication Protein A2 (RPA2) |
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| MBS4381233-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
Replication Protein A2 (RPA2) |
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| MBS4381233-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
Replication Protein A2 (RPA2) |
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| MBS4381233-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
Replication Protein A2 (RPA2) |
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| MBS4381234-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | EUR 230 |
Replication Protein A2 (RPA2) |
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| MBS4381234-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 405 |
Replication Protein A2 (RPA2) |
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| MBS4381234-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 405 |
Replication Protein A2 (RPA2) |
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| MBS4381234-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | EUR 1725 |
Replication Protein A2 (RPA2) |
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| MBS4381234-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | EUR 1725 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (APC) |
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| MBS6343183-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (APC) |
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| MBS6343183-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (FITC) |
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| MBS6343185-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (FITC) |
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| MBS6343185-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (Biotin) |
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| MBS6343184-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (Biotin) |
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| MBS6343184-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
Viral replication protein A28 |
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| MBS7043605-002mg | MyBiosource | 0.02mg | EUR 1420 |
Viral replication protein A28 |
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| MBS7043605-01mg | MyBiosource | 0.1mg | EUR 2385 |
Viral replication protein A28 |
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| MBS7043605-5x01mg | MyBiosource | 5x0.1mg | EUR 10680 |
Replication Protein A2 (RPA2) Protein |
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| abx262448-10mg | Abbexa | 10 mg | EUR 325 |
Replication Protein A2 (RPA2) Protein |
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| 20-abx262448 | Abbexa |
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Replication Protein A2 (RPA2) Protein |
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| abx262448-25mg | Abbexa | 25 mg | EUR 6575 |
Replication Protein A2 (RPA2) Protein |
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| abx262448-5mg | Abbexa | 5 mg | EUR 225 |
Replication protein 1a Antibody |
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| CSB-PA308969XA01TFN-02mg | Cusabio | 0.2mg | Ask for price |
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Description: Recombinant Tobacco streak virus (strain WC) (TSV) Replication protein 1a protein |
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Replication protein 1a Antibody |
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| CSB-PA308969XA01TFN-10mg | Cusabio | 10mg | Ask for price |
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Description: Recombinant Tobacco streak virus (strain WC) (TSV) Replication protein 1a protein |
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Replication protein 1a Antibody |
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| MBS7149763-INQUIRE | MyBiosource | INQUIRE | Ask for price |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 405) |
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| MBS6343187-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 405) |
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| MBS6343187-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 490) |
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| MBS6343188-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 490) |
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| MBS6343188-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 550) |
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| MBS6343189-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 550) |
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| MBS6343189-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 650) |
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| MBS6343190-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 650) |
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| MBS6343190-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 750) |
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| MBS6343191-01mL | MyBiosource | 0.1mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (MaxLight 750) |
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| MBS6343191-5x01mL | MyBiosource | 5x0.1mL | EUR 4250 |
RPA70, p70 (Replication Protein A, RPA1, RP-A, REPA1, HSSB, Replication Protein A1 (70kD), RP-A, HSSB, Human Single Stranded DNA-Binding Protein, RF-A, Replication Factor A) |
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| MBS600132-01mg | MyBiosource | 0.1mg | EUR 680 |
RPA70, p70 (Replication Protein A, RPA1, RP-A, REPA1, HSSB, Replication Protein A1 (70kD), RP-A, HSSB, Human Single Stranded DNA-Binding Protein, RF-A, Replication Factor A) |
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| MBS600132-5x01mg | MyBiosource | 5x0.1mg | EUR 2910 |
Plant Replication Protein A1 (RPA1) ELISA Kit |
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| MBS9370244-INQUIRE | MyBiosource | INQUIRE | Ask for price |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (Azide free) (HRP) |
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| MBS6343186-02mL | MyBiosource | 0.2mL | EUR 980 |
RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (Azide free) (HRP) |
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| MBS6343186-5x02mL | MyBiosource | 5x0.2mL | EUR 4250 |
RPA2 (Replication Protein A2, 32kD) |
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| MBS603767-01mg | MyBiosource | 0.1mg | EUR 680 |
RPA2 (Replication Protein A2, 32kD) |
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| MBS603767-5x01mg | MyBiosource | 5x0.1mg | EUR 2910 |
Recombinant RNA replication protein |
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| MBS1142041-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1085 |
Recombinant RNA replication protein |
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| MBS1142041-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 675 |
Recombinant RNA replication protein |
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| MBS1142041-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 835 |
Recombinant RNA replication protein |
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| MBS1142041-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 785 |
Recombinant RNA replication protein |
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| MBS1142041-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 980 |
RPA3 (Replication Protein A 14kD Subunit, RP-A p14, Replication Factor A Protein 3, RF-A Protein 3, REPA3, RPA14) (PE) |
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| MBS6160020-01mL | MyBiosource | 0.1(mL | EUR 875 |
Speedy and easy pressure-sensitive adhesive microdevice fabrication for sequence-specific seize and fluorescence detection of sepsis-related bacterial plasmid gene sequences
Microbial resistance to at the moment out there antibiotics poses an amazing menace within the international combat in opposition to infections. An necessary step in figuring out bacterial antibiotic resistance may be selective DNA sequence seize and fluorescence labeling. On this paper, we show the fabrication of straightforward, strong, cheap microfluidic gadgets for DNA seize and fluorescence detection of a mannequin antibiotic resistance gene sequence.
We laser micromachined polymethyl methacrylate microchannels and enclosed them utilizing pressure-sensitive adhesive tapes.
We then shaped porous polymer monoliths with DNA seize probes in these microchannels and used them for sequence-specific seize, fluorescent labeling, and laser-induced fluorescence detection of picomolar (pM) concentrations of artificial and plasmid antibiotic resistance gene targets.
The relative fluorescence for the elution peaks elevated with loaded goal DNA focus. We noticed larger fluorescence sign and % restoration for artificial goal DNA in comparison with plasmid DNA on the similar loaded goal focus. A non-target gene was used for management experiments and produced < 3% seize relative to the identical focus of the goal.
The total evaluation course of together with system fabrication was accomplished in lower than 90 min with a restrict of detection of 30 pM. The simplicity of system fabrication and good DNA seize selectivity demonstrated herein have the potential for software with processes for bacterial plasmid DNA extraction and single-particle counting to facilitate the willpower of antibiotic susceptibility. Graphical summary.
