
rmbiomed
A Standardized Inverter Package deal Borne by Broad Host Vary Plasmids for Genetic Circuit Design in Gram-Damaging Micro organism
Genetically encoded logic gates, particularly inverters-NOT gates-are the constructing blocks for designing circuits, engineering biosensors, or decision-making gadgets in artificial biology. Nonetheless, the repertoire of inverters available for various species is moderately restricted. On this work, a big complete of NOT gates that was proven to operate beforehand in a particular pressure of Escherichia coli, was recreated as broad host vary (BHR) assortment of constructs assembled in low, medium, and excessive copy quantity plasmid backbones of the SEVA (Normal European Vector Structure) assortment.
The enter/output operate of every of the gates was characterised and parametrized within the environmental bacterium and metabolic engineering chassis Pseudomonas putida. Comparisons of the ensuing fluorescence cytometry information with these printed for a similar gates in Escherichia coli supplied helpful hints on the portability of the corresponding gates.
The hereby described inverter bundle (20 totally different variations of 12 distinct gates) borne by BHR plasmids thus turns into a toolbox of selection for designing genetic circuitries in quite a lot of Gram-negative species apart from E. coli.
Formulation and characterization of nanoemulsion from Alhagi maurorum important oil and research of its antimicrobial, antibiofilm, and plasmid curing exercise in opposition to antibiotic-resistant pathogenic micro organism
Nanoemulsion expertise is an alternate candidate to beat antibiotic resistance in pathogenic micro organism. The intention of this analysis was nanoemulsion manufacturing from the important oil of Alhagi maurorum and the characterization of this nanostructure. Nanoemulsion of important oil from A. maurorum was ready utilizing the ionotropic gelation technique and chitosan as a nano-carrier. Scanning Electron Microscopy (SEM) was used to characterize the synthesized nanoparticles.
The impact of nanoemulsion on the antibacterial, antibiofilm, and plasmid curing of six antibiotic-resistant pathogenic micro organism (P. aeruginosa, E. coli, S. aureus, Okay. pneumonia, A. baumannii, B. cereus) was evaluated.
- The outcomes of this research confirmed that nanoparticles had a spherical form and easy topology. The imply dimension had been 172 ± four nm and Zeta potentials was +28.6 mv. The outcomes of antibacterial exercise confirmed that nanoemulsion of important oil had larger inhibition in opposition to micro organism in comparison with free important oil.
- Additionally, this nanoemulsion had antibiofilm exercise. Minimal Inhibitory Focus (MIC) and Minimal Bactericidal Focus for Biofilm (MBCB) had been decided for nanoemulsion in opposition to the biofilm of pathogenic micro organism.
- The outcomes have proven that the MIC worth for A. baumannii is 12.5 mg ml -1 and for E. coli this worth is 1.75 mg ml -1. This discovering implies that MIC values had been highest for A. baumannii and lowest for E. coli. Statistical evaluation demonstrated that the inhibitory impact of nanoemulsion in opposition to bacterial biofilm was important (P < 0.05).
- This nanoemulsion additionally had a exceptional impact the curing of R-plasmid of three antibiotic-resistant micro organism. Based on GC-MS evaluation of A. maurorum important oil, the principle compounds had been oxygenated sesquiterpenes and hydrocarbons. Nanoemulsion of A. maurorum had the potential to make use of as appropriate antimicrobial brokers in opposition to antibiotic-resistant micro organism.
Emergence of plasmid-mediated mcr genes from Gram-negative micro organism on the human-animal interface
Background: The worldwide emergence of plasmid-mediated colistin resistance (Col-R) conferred by mcr genes in gram-negative rods (GNRs) has jeopardized the final therapy possibility for multidrug-resistant bacterial infections in people. This research aimed to evaluate the emergence of mcr gene-mediated Col-R in GNRs remoted from people and animals in Pakistan.
Strategies: Animal and medical specimens collected from numerous sources had been prospectively analysed utilizing commonplace microbiological procedures. Pathogens had been recognized utilizing the API 20E and API 20NE methods (bioMerieux).
Minimal inhibitory focus (MIC) in opposition to colistin was decided utilizing the MIC detection strategies, and multiplex polymerase chain response (PCR) was used to amplify the mcr-1 to mcr-5 genes.
Outcomes: We remoted 126 (88.1%) animal and 17 (11.9%) human Col-R phenotypes, amongst which there was a big affiliation (P < 0.01) of Escherichia coli and Proteus mirabilis with animals and of Acinetobacter baumannii with people.
Animal strains exhibited statistically important (P < 0.05) resistance to co-trimoxazole, chloramphenicol, and moxifloxacin, and the human pathogens exhibited statistically important (P < 0.05) antibiotic resistance to cephalosporins, carbapenems, and piperacillin-tazobactam.
For Col-R strains, MIC50 values had been > 6 µg/mL and > 12 µg/mL for human and animal isolates, respectively. mcr genes had been detected in 110 (76.9%) bacterial strains, of which 108 (98.2%) had been mcr-1 and a couple of (1.8%) had been mcr-2.
Conclusions: The detection of a substantial variety of MCR-1 and MCR-2 genes in animals is worrisome, as they’re now being detected in medical pathogens. The acquisition of mcr genes by colistin-susceptible micro organism might go away us in a post-antibiotic period.
Key phrases: Antibacterial profile; Antimicrobial resistance; Colistin resistance; Cell colistin resistance; Plasmid-mediated resistance; mcr genes.
Replication Protein A1 (RPA1) Antibody |
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20-abx174378 | Abbexa |
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Replication Protein A1 (RPA1) Antibody |
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20-abx174379 | Abbexa |
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Replication Protein A1 (RPA1) Antibody |
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20-abx178250 | Abbexa |
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Replication Protein A1 (RPA1) Antibody |
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20-abx178251 | Abbexa |
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Replication Protein A1 (RPA1) Antibody |
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abx237391-100ug | Abbexa | 100 ug | EUR 610.8 |
Replication Protein A2 (RPA2) Antibody |
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abx237392-100ug | Abbexa | 100 ug | EUR 610.8 |
Replication Protein A3 (RPA3) Antibody |
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abx237393-100ug | Abbexa | 100 ug | EUR 661.2 |
Replication Protein A2 (RPA2) Antibody |
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20-abx125040 | Abbexa |
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Replication Protein A1 (RPA1) Antibody |
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abx159678-100ul | Abbexa | 100 ul | EUR 560.4 |
Replication Protein A2 (RPA2) Antibody |
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abx159679-100ul | Abbexa | 100 ul | EUR 560.4 |
Replication Protein A1 (RPA1) Antibody |
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20-abx301840 | Abbexa |
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Replication Protein A1 (RPA1) Antibody |
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abx010302-100ug | Abbexa | 100 ug | EUR 493.2 |
Replication Protein A1 (RPA1) Antibody |
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abx028041-400ul | Abbexa | 400 ul | EUR 627.6 |
Replication Protein A1 (RPA1) Antibody |
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abx028041-80l | Abbexa | 80 µl | EUR 343.2 |
Replication Protein A2 (RPA2) Antibody |
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abx331546-100ul | Abbexa | 100 ul | EUR 510 |
Human Replication Protein A1 (RPA1) Protein |
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20-abx654942 | Abbexa |
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RPA32 Antibody / Replication Protein A2 |
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RQ5919 | NSJ Bioreagents | 100 ug | EUR 356.15 |
Description: This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. |
RPA32 Antibody / Replication Protein A2 |
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V8066-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
RPA32 Antibody / Replication Protein A2 |
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V8066-20UG | NSJ Bioreagents | 20 ug | EUR 153.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
RPA32 Antibody / Replication Protein A2 |
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V8066SAF-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
RPA32 Antibody / Replication Protein A2 |
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V7392-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. [UniProt] |
RPA32 Antibody / Replication Protein A2 |
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V7392-20UG | NSJ Bioreagents | 20 ug | EUR 153.3 |
Description: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. [UniProt] |
RPA32 Antibody / Replication Protein A2 |
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V7392SAF-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. [UniProt] |
RPA32 Antibody / Replication Protein A2 |
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V7886-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
RPA32 Antibody / Replication Protein A2 |
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V7886-20UG | NSJ Bioreagents | 20 ug | EUR 153.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
RPA32 Antibody / Replication Protein A2 |
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V7886SAF-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
RPA32 Antibody / Replication Protein A2 |
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V7341-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
RPA32 Antibody / Replication Protein A2 |
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V7341-20UG | NSJ Bioreagents | 20 ug | EUR 153.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
RPA32 Antibody / Replication Protein A2 |
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V7341IHC-7ML | NSJ Bioreagents | 7 ml | EUR 349.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
RPA32 Antibody / Replication Protein A2 |
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V7341SAF-100UG | NSJ Bioreagents | 100 ug | EUR 349.3 |
Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. |
Replication Protein A2 (RPA2) ELISA Kit |
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abx595526-96tests | Abbexa | 96 tests | EUR 764.4 |
Replication Protein A1, 70 kDa Antibody |
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20-abx115128 | Abbexa |
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Replication Protein A2, 32 kDa Antibody |
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20-abx115129 | Abbexa |
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Replication Protein A4, 34 kDa Antibody |
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20-abx115130 | Abbexa |
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Rat Replication Protein A1 (RPA1) CLIA Kit |
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20-abx495409 | Abbexa |
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Recombinant Human Replication Protein A2 |
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7-06178 | CHI Scientific | 2µg | Ask for price |
Recombinant Human Replication Protein A2 |
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7-06179 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Replication Protein A2 |
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7-06180 | CHI Scientific | 1mg | Ask for price |
Replication Protein A1 (RPA1) Antibody (HRP) |
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20-abx315981 | Abbexa |
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Rat Replication Protein A1 (RPA1) ELISA Kit |
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DLR-RPA1-Ra-48T | DL Develop | 48T | EUR 658.8 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Replication Protein A1 (RPA1) in samples from tissue homogenates, cell lysates or other biological fluids. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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DLR-RPA1-Ra-96T | DL Develop | 96T | EUR 861.6 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Replication Protein A1 (RPA1) in samples from tissue homogenates, cell lysates or other biological fluids. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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20-abx156054 | Abbexa |
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Pig Replication Protein A1 (RPA1) ELISA Kit |
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abx360874-96tests | Abbexa | 96 tests | EUR 990 |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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RD-RPA1-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 668.4 |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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RD-RPA1-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 930 |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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RDR-RPA1-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 699.6 |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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RDR-RPA1-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 973.2 |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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SEH217Ra-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6149.04 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Replication Protein A1 (RPA1) in Tissue homogenates, cell lysates and other biological fluids. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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SEH217Ra-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 611.57 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Replication Protein A1 (RPA1) in Tissue homogenates, cell lysates and other biological fluids. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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SEH217Ra-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 822.24 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Replication Protein A1 (RPA1) in Tissue homogenates, cell lysates and other biological fluids. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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SEH217Ra-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3340.08 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Replication Protein A1 (RPA1) in Tissue homogenates, cell lysates and other biological fluids. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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4-SEH217Ra | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Replication Protein A1 (RPA1) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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RD-RPA1-Ra-48T | Reddot Biotech | 48T | EUR 487.7 |
Description: tissue homogenates, cell lysates and other biological fluids. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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RD-RPA1-Ra-96T | Reddot Biotech | 96T | EUR 696.7 |
Description: tissue homogenates, cell lysates and other biological fluids. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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RDR-RPA1-Ra-48T | Reddot Biotech | 48T | EUR 512.09 |
Description: tissue homogenates, cell lysates and other biological fluids. |
Rat Replication Protein A1 (RPA1) ELISA Kit |
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RDR-RPA1-Ra-96T | Reddot Biotech | 96T | EUR 731.54 |
Description: tissue homogenates, cell lysates and other biological fluids. |
Replication Protein A1 (RPA1) Antibody (FITC) |
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20-abx315982 | Abbexa |
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Replication Protein A2 (RPA2) Antibody Pair |
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abx117402-1pair5x96wellplates | Abbexa | 1 pair (5x96 well plates) | EUR 1212 |
Human Replication Protein A1 (RPA1) CLIA Kit |
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20-abx495408 | Abbexa |
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RPA32 Antibody / RPA2 / Replication Protein A2 |
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RQ6589 | NSJ Bioreagents | 100ug | EUR 356.15 |
Description: This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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20-abx152981 | Abbexa |
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Human Replication Protein A2 (RPA2) ELISA Kit |
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abx382883-96tests | Abbexa | 96 tests | EUR 1093.2 |
Human Replication Protein A3 (RPA3) ELISA Kit |
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abx382884-96tests | Abbexa | 96 tests | EUR 1093.2 |
Human Replication Protein A1 (RPA1) ELISA Kit |
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DLR-RPA1-Hu-48T | DL Develop | 48T | EUR 620.4 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Replication Protein A1 (RPA1) in samples from tissue homogenates or other biological fluids. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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DLR-RPA1-Hu-96T | DL Develop | 96T | EUR 807.6 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Replication Protein A1 (RPA1) in samples from tissue homogenates or other biological fluids. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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abx573009-96tests | Abbexa | 96 tests | EUR 943.2 |
Human Replication Protein A1 (RPA1) ELISA Kit |
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RD-RPA1-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 625.2 |
Human Replication Protein A1 (RPA1) ELISA Kit |
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RD-RPA1-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 867.6 |
Human Replication Protein A1 (RPA1) ELISA Kit |
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RDR-RPA1-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 652.8 |
Human Replication Protein A1 (RPA1) ELISA Kit |
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RDR-RPA1-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 907.2 |
Human Replication Protein A1 (RPA1) ELISA Kit |
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SEH217Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5677.8 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Replication Protein A1 (RPA1) in Tissue homogenates and other biological fluids. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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SEH217Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 572.76 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Replication Protein A1 (RPA1) in Tissue homogenates and other biological fluids. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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SEH217Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 766.8 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Replication Protein A1 (RPA1) in Tissue homogenates and other biological fluids. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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SEH217Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3090.6 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Replication Protein A1 (RPA1) in Tissue homogenates and other biological fluids. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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4-SEH217Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Replication Protein A1 (RPA1) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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RD-RPA1-Hu-48T | Reddot Biotech | 48T | EUR 452.7 |
Description: tissue homogenates and other biological fluids. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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RD-RPA1-Hu-96T | Reddot Biotech | 96T | EUR 646.7 |
Description: tissue homogenates and other biological fluids. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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RDR-RPA1-Hu-48T | Reddot Biotech | 48T | EUR 475.34 |
Description: tissue homogenates and other biological fluids. |
Human Replication Protein A1 (RPA1) ELISA Kit |
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RDR-RPA1-Hu-96T | Reddot Biotech | 96T | EUR 679.04 |
Description: tissue homogenates and other biological fluids. |
Replication Protein A1 (RPA1) Antibody (Biotin) |
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20-abx315983 | Abbexa |
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Monkey Replication Protein A1 (RPA1) ELISA Kit |
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abx358961-96tests | Abbexa | 96 tests | EUR 990 |
Rabbit Replication Protein A1 (RPA1) ELISA Kit |
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abx363576-96tests | Abbexa | 96 tests | EUR 990 |
ELISA kit for Rat RPA1 (Replication Protein A1) |
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ELK6959 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Replication Protein A1 from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Replication Initiation Protein (repD) Antibody |
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20-abx300249 | Abbexa |
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Chicken Replication Protein A1 (RPA1) ELISA Kit |
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abx355854-96tests | Abbexa | 96 tests | EUR 990 |
Replication protein A 32 kDa subunit Antibody |
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20-abx109440 | Abbexa |
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Replication protein A 14 kDa subunit Antibody |
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20-abx110173 | Abbexa |
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Replication protein A 14 kDa subunit Antibody |
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20-abx110174 | Abbexa |
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Replication protein A 30 kDa subunit Antibody |
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20-abx110175 | Abbexa |
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Human replication protein A,RPA-70 ELISA Kit |
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201-12-1914 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human replication protein A,RPA-70 ELISA Kit |
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CN-04330H1 | ChemNorm | 96T | EUR 538.8 |
Human replication protein A,RPA-70 ELISA Kit |
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CN-04330H2 | ChemNorm | 48T | EUR 358.8 |
ELISA kit for Human RPA1 (Replication Protein A1) |
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ELK4413 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Replication Protein A1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
ELISA kit for Human RPA1 (Replication protein A1) |
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E-EL-H1282 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
Description: A sandwich ELISA kit for quantitative measurement of Human RPA1 (Replication protein A1) in samples from Serum, Plasma, Cell supernatant |
Human replication protein A(RPA-70)ELISA Kit |
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GA-E1930HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human replication protein A(RPA-70)ELISA Kit |
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GA-E1930HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Human replication protein A(RPA-70)ELISA Kit |
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QY-E03530 | Qayee Biotechnology | 96T | EUR 433.2 |
Human replication protein A,RPA-70 ELISA Kit |
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YLA2844HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human replication protein A,RPA-70 ELISA Kit |
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YLA2844HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Human Replication protein A 14KDA subunit (RPA3) |
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1-CSB-RP162344h | Cusabio |
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Description: Recombinant Human Replication protein A 14KDA subunit(RPA3),partial expressed in E.coli |
Human Replication protein A 14KDA subunit (RPA3) |
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1-CSB-RP162394h | Cusabio |
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Description: Recombinant Human Replication protein A 14KDA subunit(RPA3),partial expressed in E.coli |
Human Replication protein A 30KDA subunit (RPA4) |
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1-CSB-RP162494h | Cusabio |
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Description: Recombinant Human Replication protein A 30KDA subunit(RPA4),partial expressed in E.coli |
Human Replication protein A 32KDA subunit (RPA2) |
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1-CSB-RP015554h | Cusabio |
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Description: Recombinant Human Replication protein A 32KDA subunit(RPA2),partial expressed in E.coli |
Human Replication Initiator 1 (REPIN1) Protein |
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20-abx168192 | Abbexa |
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Mouse Replication Initiator 1 (REPIN1) Protein |
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20-abx168411 | Abbexa |
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Replication protein A2 (RPA2) polyclonal antibody |
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ABP-PAB-01174 | Allele Biotech | 100 ug | Ask for price |
RPA2 Replication Protein A2 Human Recombinant Protein |
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PROTP15927 | BosterBio | Regular: 10ug | EUR 380.4 |
Description: RPA2 Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 293 amino acids (1-270 a.a.) and having a molecular mass of 31.7kDa. The RPA2 is purified by proprietary chromatographic techniques. |
Replication Initiation Protein (REPD) Antibody (HRP) |
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20-abx300250 | Abbexa |
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Replication protein A 32 kDa subunit Antibody (HRP) |
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20-abx107841 | Abbexa |
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Replication protein A 14 kDa subunit Antibody (HRP) |
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20-abx108713 | Abbexa |
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Replication protein A 14 kDa subunit Antibody (HRP) |
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20-abx108714 | Abbexa |
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Replication protein A 30 kDa subunit Antibody (HRP) |
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20-abx108715 | Abbexa |
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Replication Initiation Protein (REPD) Antibody (FITC) |
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20-abx300251 | Abbexa |
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Replication protein A 32 kDa subunit Antibody (FITC) |
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20-abx106426 | Abbexa |
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Replication protein A 14 kDa subunit Antibody (FITC) |
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20-abx107293 | Abbexa |
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Speedy and easy pressure-sensitive adhesive microdevice fabrication for sequence-specific seize and fluorescence detection of sepsis-related bacterial plasmid gene sequences
Microbial resistance to at the moment out there antibiotics poses an amazing menace within the international combat in opposition to infections. An necessary step in figuring out bacterial antibiotic resistance may be selective DNA sequence seize and fluorescence labeling. On this paper, we show the fabrication of straightforward, strong, cheap microfluidic gadgets for DNA seize and fluorescence detection of a mannequin antibiotic resistance gene sequence.
We laser micromachined polymethyl methacrylate microchannels and enclosed them utilizing pressure-sensitive adhesive tapes.
We then shaped porous polymer monoliths with DNA seize probes in these microchannels and used them for sequence-specific seize, fluorescent labeling, and laser-induced fluorescence detection of picomolar (pM) concentrations of artificial and plasmid antibiotic resistance gene targets.
The relative fluorescence for the elution peaks elevated with loaded goal DNA focus. We noticed larger fluorescence sign and % restoration for artificial goal DNA in comparison with plasmid DNA on the similar loaded goal focus. A non-target gene was used for management experiments and produced < 3% seize relative to the identical focus of the goal.
The total evaluation course of together with system fabrication was accomplished in lower than 90 min with a restrict of detection of 30 pM. The simplicity of system fabrication and good DNA seize selectivity demonstrated herein have the potential for software with processes for bacterial plasmid DNA extraction and single-particle counting to facilitate the willpower of antibiotic susceptibility. Graphical summary.