RM and Biomed LTD. Physiology Bioresonance Monoclonal,Polyclonal,rna DNA-binding site-dependent foci within the bacterial cytoplasm

DNA-binding site-dependent foci within the bacterial cytoplasm


rmbiomed

VirB, a key transcriptional regulator of virulence plasmid genes in Shigella flexneri, varieties DNA-binding site-dependent foci within the bacterial cytoplasm

VirB is a key regulator of genes situated on the massive virulence plasmid (pINV) within the bacterial pathogen Shigella flexneri VirB is uncommon; it’s not associated to different transcriptional regulators, as an alternative, it belongs to a household of proteins that primarily operate in plasmid and chromosome partitioning; exemplified by ParB. Regardless of this, VirB doesn’t operate to segregate DNA, however moderately counters transcriptional silencing mediated by the nucleoid structuring protein, H-NS.
Since ParB localizes subcellularly as discrete foci within the bacterial cytoplasm, we selected to analyze the subcellular localization of VirB to realize novel perception into how VirB capabilities as a transcriptional anti-silencer.
To do that, a GFP-VirB fusion that retains the regulatory exercise of VirB and but, doesn’t bear important protein degradation in S. flexneri, was used. Surprisingly, discrete fluorescent foci had been noticed in reside wild-type S. flexneri cells and an isogenic virB mutant utilizing fluorescence microscopy.
In distinction, foci had been hardly ever noticed (<10%) in pINV-cured cells or in cells expressing a GFP-VirB fusion carrying amino acid substitutions within the VirB DNA binding area. Lastly, the 25 bp VirB-binding web site was demonstrated to be enough and vital for GFP-VirB focus formation utilizing a set of small surrogate plasmids.
Mixed, these information show that the VirB:DNA interactions required for the transcriptional anti-silencing exercise of VirB on pINV are a prerequisite for the subcellular localization of VirB within the bacterial cytoplasm. The importance of those findings, in gentle of the anti-silencing exercise of VirB, is mentioned.
SignificanceThis research reveals the subcellular localization of VirB, a key transcriptional regulator of virulence genes discovered on the massive virulence plasmid (pINV) in Shigella. Fluorescent alerts generated by an energetic GFP-VirB fusion type 2, 3, or Four discrete foci within the bacterial cytoplasm, predominantly on the quarter cell place. These alerts are fully dependent upon VirB interacting with its DNA binding web site discovered both on the virulence plasmid or an engineered surrogate.
Our findings:
1) present novel perception into VirB:pINV interactions,
2) counsel that VirB might have utility as a DNA marker, and
3) elevate questions on how and why this anti-silencing protein controls virulence gene expression on pINV of Shigella spp. varieties discrete foci/hubs inside the bacterial cytoplasm.
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Escherichia coli Outer membrane protein A (ompA)

1-CSB-CF364256ENV(A4)
  • EUR 1560.00
  • EUR 688.80
  • EUR 996.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Escherichia coli Outer membrane protein A(ompA) expressed in in vitro E.coli expression system

Escherichia coli F17 fimbrial protein (F17a-A)

1-CSB-EP318126ENL
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  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli F17 fimbrial protein(F17a-A) expressed in E.coli

Escherichia coli Lipoate-protein ligase A (lplA)

1-CSB-EP510777ENU
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  • EUR 1461.60
  • EUR 476.40
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Description: Recombinant Escherichia coli Lipoate-protein ligase A(lplA) expressed in E.coli

Escherichia coli Glyoxylate/hydroxypyruvate reductase A (ghrA)

1-CSB-EP482379ENM
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  • EUR 1461.60
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Description: Recombinant Escherichia coli Glyoxylate/hydroxypyruvate reductase A(ghrA) expressed in E.coli

Escherichia coli Glyoxylate/hydroxypyruvate reductase A (ghrA)

1-CSB-EP482379ENMe1
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  • EUR 1602.00
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Description: Recombinant Escherichia coli Glyoxylate/hydroxypyruvate reductase A(ghrA) expressed in E.coli

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abx415692-01mg 0.1 mg
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A-4010
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A-4012
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Histone H3K9ac (Acetyl H3K9) Polyclonal Antibody

A-4022
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Histone H3K14ac (Acetyl H3K14) Polyclonal Antibody

A-4023
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Histone H3K18ac (Acetyl H3K18) Polyclonal Antibody

A-4024
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Histone H3K23ac (Acetyl H3K23) Polyclonal Antibody

A-4025
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Histone H3K56ac (Acetyl H3K56) Polyclonal Antibody

A-4026
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Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody

A-4031
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Histone H3K4me2 (H3K4 Dimethyl) Polyclonal Antibody

A-4032
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Histone H3K4me3 (H3K4 Trimethyl) Polyclonal Antibody

A-4033
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Histone H3K9me1 (H3K9 Monomethyl) Polyclonal Antibody

A-4034
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Histone H3K9me2 (H3K9 Dimethyl) Polyclonal Antibody

A-4035
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Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody

A-4036
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  • EUR 336.60
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Histone H3K27me1 (H3K27 Monomethyl) Polyclonal Antibody

A-4037
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Histone H3K27me2 (H3K27 Dimethyl) Polyclonal Antibody

A-4038
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Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody

A-4039
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Histone H3K36me1 (H3K36 Monomethyl) Polyclonal Antibody

A-4040
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Histone H3K36me2 (H3K36 Dimethyl) Polyclonal Antibody

A-4041
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Histone H3K36me3 (H3K36 Trimethyl) Polyclonal Antibody

A-4042
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  • EUR 336.60
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Histone H3K79me1 (H3K79 Monomethyl) Polyclonal Antibody

A-4043
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Histone H3K79me2 (H3K79 Dimethyl) Polyclonal Antibody

A-4044
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Histone H3K79me3 (H3K79 Trimethyl) Polyclonal Antibody

A-4045
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Histone H4K20me1 (H4K20 Monomethyl) Polyclonal Antibody

A-4046
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  • EUR 470.80
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Histone H4K20me2 (H4K20 Dimethyl) Polyclonal Antibody

A-4047
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  • EUR 470.80
  • 50 µl
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Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody

A-4048
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  • EUR 173.80
  • EUR 302.50
  • EUR 470.80
  • 50 µl
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Histone H3K27ac (Acetyl H3K27) Polyclonal Antibody

A-4708
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  • EUR 173.80
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  • EUR 510.40
  • 100 µl
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CRISPR Cas9 Monoclonal Antibody [7A9]

A-9000
  • EUR 573.30
  • EUR 71.50
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  • EUR 355.30
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CRISPR/Cas9 (SaCas9) Monoclonal Antibody [6H4]

A-9001
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  • EUR 70.40
  • EUR 192.50
  • EUR 338.80
  • 100 µg
  • 10 ug
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Escherichia coli Protein (ECP) Protein

20-abx066464
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
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  • 1 mg
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  • 50 ug

CLIMP-63 Polyclonal Antibody

A-0701
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DNMT3A Polyclonal Antibody

A-1003
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  • EUR 235.40
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DNMT3B Polyclonal Antibody

A-1004
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DNMT3L Polyclonal Antibody

A-1005
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MBD3 Polyclonal Antibody

A-1008
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MGMT Polyclonal Antibody

A-1010
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MeCP2 Polyclonal Antibody

A-1012
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TET1 Polyclonal Antibody

A-1020
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DNMT1 Polyclonal Antibody

A-1700
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TET2 Polyclonal Antibody

A-1701
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MBD2 Polyclonal Antibody

A-1713
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PRDM2 Polyclonal Antibody

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PRDM3 Polyclonal Antibody

A-2003
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PRDM6 Polyclonal Antibody

A-2006
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PRDM12 Polyclonal Antibody

A-2012
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A-2014
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EZH2 Polyclonal Antibody

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RNA Polymerase II Monoclonal Antibody [CTD4H8]

A-2032
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CBX5 Polyclonal Antibody

A-2701
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EGLN1 Polyclonal Antibody

A-2702
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RBBP4 Polyclonal Antibody

A-2703
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SIN3A Polyclonal Antibody

A-2704
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CTBP1 Polyclonal Antibody

A-2705
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PADI4 Polyclonal Antibody

A-2706
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A-2707
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IDH1 Polyclonal Antibody

A-2708
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CHD4 Polyclonal Antibody

A-2709
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CHAF1A Polyclonal Antibody

A-2710
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BRD7 Polyclonal Antibody

A-2712
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UHRF1 Polyclonal Antibody

A-2714
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  • EUR 223.30
  • EUR 353.10
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  • 100 ul

UHRF2 Polyclonal Antibody

A-2715
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  • EUR 353.10
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  • 100 ul

SMARCE1 Polyclonal Antibody

A-2716
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  • EUR 223.30
  • EUR 353.10
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ACTL6A Polyclonal Antibody

A-2717
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  • EUR 353.10
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  • 100 ul

MPG Polyclonal Antibody

A-2718
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  • EUR 353.10
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HIF1AN Polyclonal Antibody

A-2719
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  • EUR 223.30
  • EUR 353.10
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  • 100 ul

RNF2 Polyclonal Antibody

A-2720
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  • EUR 223.30
  • EUR 353.10
  • 100 µl
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  • 100 ul

PCGF6 Polyclonal Antibody

A-2721
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCB1 Polyclonal Antibody

A-2722
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

ALKBH3 Polyclonal Antibody

A-2723
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

BBOX1 Polyclonal Antibody

A-2724
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

BTAF1 Polyclonal Antibody

A-2725
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

CXXC1 Polyclonal Antibody

A-2726
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

KDM4B Polyclonal Antibody

A-2727
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

HELLS Polyclonal Antibody

A-2728
  • EUR 742.08
  • EUR 303.60
  • EUR 463.10
  • 100 µl
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  • 100 ul

ING3 Polyclonal Antibody

A-2729
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

ING4 Polyclonal Antibody

A-2730
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

PHC1 Polyclonal Antibody

A-2731
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCAD1 Polyclonal Antibody

A-2732
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

USP16 Polyclonal Antibody

A-2733
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCC1 Polyclonal Antibody

A-2734
  • EUR 742.08
  • EUR 303.60
  • EUR 463.10
  • 100 µl
  • 50 ul
  • 100 ul

ERCC6L Polyclonal Antibody

A-2735
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMYD5 Polyclonal Antibody

A-2736
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

ATF2 Polyclonal Antibody

A-2738
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

BMI1 Polyclonal Antibody

A-2739
  • EUR 869.10
  • Ask for price
  • Ask for price
  • 100 µl
  • 50 ul
  • 100 ul

CTBP2 Polyclonal Antibody

A-2740
  • EUR 869.10
  • EUR 322.30
  • EUR 548.90
  • 100 µl
  • 50 ul
  • 100 ul

CTCF Polyclonal Antibody

A-2741
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

MTA2 Polyclonal Antibody

A-2742
  • EUR 869.10
  • EUR 322.30
  • EUR 548.90
  • 100 µl
  • 50 ul
  • 100 ul

PMS1 Polyclonal Antibody

A-2744
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SFN Polyclonal Antibody

A-2745
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCA4 Polyclonal Antibody

A-2746
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCAL1 Polyclonal Antibody

A-2747
  • EUR 869.10
  • Ask for price
  • Ask for price
  • 100 µl
  • 50 ul
  • 100 ul

SMARCC2 Polyclonal Antibody

A-2748
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

MuERVL-Gag Polyclonal Antibody

A-2801
  • EUR 684.66
  • EUR 239.80
  • EUR 432.30
  • 100 µl
  • 50 ul
  • 100 ul

PRMT1 Polyclonal Antibody

A-3001
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

PRMT2 Polyclonal Antibody

A-3002
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

PRMT4 Polyclonal Antibody

A-3004
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

PRMT5 Polyclonal Antibody

A-3005
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

PRMT7 Polyclonal Antibody

A-3007
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

SUV39H2 Polyclonal Antibody

A-3010
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

SET9 Polyclonal Antibody

A-3014
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

CBX4 Polyclonal Antibody

A-3016
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 50 ul
  • 100 ul

CBX8 Polyclonal Antibody

A-3017
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 50 ul
  • 100 ul

EHMT2 Polyclonal Antibody

A-3019
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

SETDB1 Polyclonal Antibody

A-3700
  • EUR 535.02
  • Ask for price
  • Ask for price
  • 50 µl
  • 50 ul
  • 100 ul

KAT5 Polyclonal Antibody

A-3701
  • EUR 535.02
  • Ask for price
  • Ask for price
  • 50 µl
  • 50 ul
  • 100 ul

SETD1B Polyclonal Antibody

A-3702
  • EUR 535.02
  • EUR 322.30
  • EUR 423.50
  • 50 µl
  • 50 ul
  • 100 ul

KDM3B Polyclonal Antibody

A-3703
  • EUR 535.02
  • EUR 322.30
  • EUR 470.80
  • 50 µl
  • 50 ul
  • 100 ul

KDM3A Polyclonal Antibody

A-3704
  • EUR 535.02
  • EUR 322.30
  • EUR 423.50
  • 50 µl
  • 50 ul
  • 100 ul

Histone H3R2 Dimethyl Symmetric (H3R2me2s) Polyclonal Antibody

A-3705
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R8 Dimethyl Symmetric (H3R8me2s) Polyclonal Antibody

A-3706
  • EUR 754.26
  • EUR 304.70
  • EUR 479.60
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R26 Dimethyl Asymmetric (H3R26me2a) Polyclonal Antibody

A-3707
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H4R3 Dimethyl Asymmetric (H4R3me2a) Polyclonal Antibody

A-3708
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody

A-3709
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R17 Monomethyl (H3R17me1) Polyclonal Antibody

A-3710
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R17 Dimethyl Symmetric (H3R17me2s) Polyclonal Antibody

A-3711
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R26 Dimethyl Symmetric (H3R26me2s) Polyclonal Antibody

A-3712
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R2 Monomethyl (H3R2me1) Polyclonal Antibody

A-3713
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R2 Dimethyl Asymmetric (H3R2me2a) Polyclonal Antibody

A-3714
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R8 Monomethyl (H3R8me1) Polyclonal Antibody

A-3715
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R8 Dimethyl Asymmetric (H3R8me2a) Polyclonal Antibody

A-3716
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H4R3 Monomethyl (H4R3me1) Polyclonal Antibody

A-3717
  • EUR 754.26
  • EUR 299.20
  • EUR 383.90
  • 100 µg
  • 50 ul
  • 100 ul

Histone H4R3 Dimethyl Symmetric (H4R3me2s) Polyclonal Antibody

A-3718
  • EUR 754.26
  • EUR 304.70
  • EUR 479.60
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3R26 Monomethyl (H3R26me1) Polyclonal Antibody

A-3719
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

SETD2 Polyclonal Antibody

A-3720
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3K14me1 (H3K14 Monomethyl) Polyclonal Antibody

A-3721
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3K14me2 (H3K14 Dimethyl) Polyclonal Antibody

A-3722
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

Histone H3K14me3 (H3K14 Trimethyl) Polyclonal Antibody

A-3723
  • EUR 754.26
  • EUR 299.20
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

JMJD6 Polyclonal Antibody

A-3724
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

Pan Kme2 (Dimethyl Lysine) Polyclonal Antibody

A-3725
  • EUR 1034.40
  • EUR 353.10
  • EUR 661.10
  • 100 µl
  • 50 ul
  • 100 ul

MLL5 Polyclonal Antibody

A-3726
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

ASH2L Polyclonal Antibody

A-3727
  • EUR 580.26
  • Ask for price
  • Ask for price
  • 100 µl
  • 50 ul
  • 100 ul

KDM1A Polyclonal Antibody

A-3728
  • EUR 742.08
  • EUR 303.60
  • EUR 463.10
  • 100 µl
  • 50 ul
  • 100 ul

MBD5 Polyclonal Antibody

A-3729
  • EUR 1206.66
  • EUR 416.90
  • EUR 777.70
  • 150 µl
  • 50 ul
  • 150 ul

HDAC3 Monoclonal Antibody [A10B1]

A-4003
  • EUR 740.34
  • Ask for price
  • Ask for price
  • 100 µl
  • 50 ul
  • 100 ul

HDAC4 Polyclonal Antibody

A-4004
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

HDAC5 Polyclonal Antibody

A-4005
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

HDAC6 Polyclonal Antibody

A-4006
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

HDAC7 Polyclonal Antibody

A-4007
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

Simplified plasmid cloning with a common MCS design and bacterial in vivo meeting

Background: The power to clone DNA sequences shortly and exactly into plasmids is important for molecular biology research. The latest improvement of seamless cloning applied sciences has made important enhancements in plasmid development, however easy and dependable instruments are at all times fascinating for time- and labor-saving functions.
Outcomes: We developed and standardized a plasmid cloning protocol primarily based on a common MCS (A number of Cloning Web site) design and bacterial in vivo meeting. With this technique, the vector is linearized first by PCR (Polymerase Chain Response) or restriction digestion.
Then a small quantity (10 ~ 20 ng) of this linear vector could be blended with a PCR-amplified insert (5× molar ratio towards vector) and remodeled straight into competent E. coli cells to acquire the specified clones by means of in vivo meeting.
Since we used a 36-bp common MCS because the homologous linker, any PCR-amplified insert with ~ 15 bp appropriate termini could be cloned into the vector with excessive constancy and effectivity.
Thus, the necessity for redesigning insert-amplifying primers in keeping with varied vector sequences and the next PCR procedures was eradicated.
Conclusions: Our protocol considerably diminished hands-on time for making ready transformation reactions, had wonderful reliability, and was confirmed to be a fast and versatile plasmid cloning approach. The protocol incorporates principally mixing steps, making it an especially automation-friendly and promising software in trendy biology research.
Key phrases: Bacterial in vivo meeting; DNA meeting; Homologous sequence; Plasmid cloning; Common MCS.

A ProQ/FinO household protein concerned in plasmid copy quantity management favours health of micro organism carrying mcr-1-bearing IncI2 plasmids

The plasmid-encoded colistin resistance gene mcr-1 challenges the usage of polymyxins and poses a menace to public well being. Though IncI2-type plasmids are the commonest vector for spreading the mcr-1 gene, the mechanisms by which these plasmids adapt to host micro organism and preserve resistance genes stay unclear.
  • Herein, we investigated the regulatory mechanism for controlling the health value of an IncI2 plasmid carrying mcr-1.
  • A putative ProQ/FinO household protein encoded by the IncI2 plasmid, designated as PcnR (plasmid copy quantity repressor), balances the mcr-1 expression and micro organism health by repressing the plasmid copy quantity.
  • It binds to the primary stem-loop construction of the repR mRNA to repress RepA expression, which differs from some other beforehand reported plasmid replication management mechanism.
  • Plasmid invasion experiments revealed that pcnR is important for the persistence of the mcr-1-bearing IncI2 plasmid within the bacterial populations. Moreover, single-copy mcr-1 gene nonetheless exerted a health value to host micro organism, and negatively affected the persistence of the IncI2 plasmid in aggressive co-cultures.
  • These findings show that sustaining mcr-1 plasmid at a single copy is important for its persistence, and clarify the considerably diminished prevalence of mcr-1 following the ban of colistin as a progress promoter in China.

Bacillus thuringiensis,BT ELISA Kit

CN-00140O2 48T
EUR 387.6

Inactivated Bacillus Thuringiensis Cry1Ab Toxin

VAng-Lsx1536-10g 10 µg
EUR 2138.4
Description: Bacillus Thuringiensis Cry1Ab Toxin, native protein form Bacillus Thuringiensis

Bacillus thuringiensis subsp.Kurstaki Pesticidal crystal protein cry2Ab (cry2Ab)

1-CSB-EP324157BDC
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp.Kurstaki Pesticidal crystal protein cry2Ab(cry2Ab) expressed in E.coli

Bacillus thuringiensis subsp. Pesticidal crystal protein cry1Ac (cry1Ac)

1-CSB-YP356448BDC
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp. Pesticidal crystal protein cry1Ac(cry1Ac),partial expressed in Yeast

Bacillus thuringiensis subsp. Pesticidal crystal protein cry3Aa (cry3Aa)

1-CSB-YP358337BDG
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp. Pesticidal crystal protein cry3Aa(cry3Aa) expressed in Yeast

Bacillus thuringiensis subsp. Pesticidal crystal protein cry1Ab (cry1Ab)

1-CSB-YP363358BDC
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp. Pesticidal crystal protein cry1Ab(cry1Ab),partial expressed in Yeast

Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac (cry1Ac)

1-CSB-EP356448BDC
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac(cry1Ac),partial expressed in E.coli

Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa (cry3Aa)

1-CSB-EP358337BDG
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa(cry3Aa) expressed in E.coli

Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa (cry3Aa)

1-CSB-EP358337BDGa1
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa(cry3Aa) expressed in E.coli

Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ab (cry1Ab)

1-CSB-EP363358BDC
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ab(cry1Ab),partial expressed in E.coli

Bacillus thuringiensis subsp. morrisoni Pesticidal crystal protein cry1Fb (cry1Fb)

1-CSB-EP528987BRS
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp. morrisoni Pesticidal crystal protein cry1Fb(cry1Fb),partial expressed in E.coli

Bacillus thuringiensis subsp. morrisoni Pesticidal crystal protein Cry1Fb (cry1Fb)

1-CSB-EP528987BRSa0
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus thuringiensis subsp. morrisoni Pesticidal crystal protein Cry1Fb(cry1Fb),partial expressed in E.coli

PFU enzyme Plasmid

PVT7002 2 ug
EUR 614.4

Recombinant Bacillus Thermolysin Protein

VAng-Lsx1547-inquire inquire Ask for price
Description: Bacillus Thermolysin, recombinant protein from Bacillus.

Recombinant Bacillus Anthracis Protective Proein

VAng-Lsx1537-50g 50 µg
EUR 643.2
Description: Bacillus Anthracis Protective Protein, recombinant protein from P. fluorescens.

Recombinant Bacillus Anthracis Edema Factor

VAng-Lsx1538-100g 100 µg
EUR 537.6
Description: Bacillus Anthracis Edema Factor, recombinant protein.

Recombinant Bacillus Anthracis Lethal Factor

VAng-Lsx1540-1mg 1 mg
EUR 2370
Description: Bacillus Anthracis Lethal Factor, recombinant protein.

Recombinant Bacillus Thermoglucosidasius groL Protein

VAng-Lsx1544-inquire inquire Ask for price
Description: Bacillus thermoglucosidasius 60 kDa chaperonin], recombinant protein.

Recombinant Bacillus Thermoglucosidasius dnaK Protein

VAng-Lsx1545-inquire inquire Ask for price
Description: Bacillus thermoglucosidasius Chaperone protein DnaK, recombinant protein.

Bacillus Spores Antibody

abx022927-1ml 1 ml
EUR 718.8

BACILLUS CEREUS AGAR

B02-101-10kg 10 kg
EUR 2187.6

BACILLUS CEREUS AGAR

B02-101-2Kg 2 Kg
EUR 523.2

BACILLUS CEREUS AGAR

B02-101-500g 500 g
EUR 186

Bacillus cereus Enterotoxin

1-CSB-EP302168BQJ
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus cereus Enterotoxin,partial expressed in E.coli

Bacillus amyloliquefaciens Barstar

1-CSB-EP320008BQB
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus amyloliquefaciens Barstar expressed in E.coli

Bacillus sp. Levanase

1-CSB-EP523248BRG
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus sp. Levanase,partial expressed in E.coli

Glycine Oxidase H244K, Bacillus subtilis recombinant

7845-1000
EUR 2140.8

Glycine Oxidase H244K, Bacillus subtilis recombinant

7845-250
EUR 705.6

Recombinant Bacillus Circulans Intein Protein [His]

VAng-Lsx1542-100g 100 µg
EUR 1084.8
Description: Bacillus Circulans Intein [His], recombinant protein.

Recombinant Bacillus Circulans Intein Protein [His]

VAng-Lsx1542-500g 500 µg
EUR 2432.4
Description: Bacillus Circulans Intein [His], recombinant protein.

pET28a- ULP (SUMO enzyme) Plasmid

PVT7015 2 ug
EUR 614.4

Recombinant tTG Enzyme

abx070024-100ug 100 ug
EUR 526.8

HRASLS3 Enzyme (Recombinant)

20-abx073048
  • EUR 276.00
  • EUR 2798.40
  • EUR 393.60
  • 10 ug
  • 1 mg
  • 50 ug

AHCY Enzyme (Recombinant)

20-abx073135
  • EUR 3433.20
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

HTATIP2 Enzyme (Recombinant)

20-abx073312
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ADPRHL2 Enzyme (Recombinant)

20-abx073359
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ARSG Enzyme (Recombinant)

20-abx073379
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ARSA Enzyme (Recombinant)

20-abx073395
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

B3GAT3 Enzyme (Recombinant)

20-abx073397
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

GLUD1 Enzyme (Recombinant)

20-abx073437
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

TPO Enzyme (Recombinant)

20-abx073668
  • EUR 6674.40
  • EUR 376.80
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

ADA Enzyme (Recombinant)

20-abx073712
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

TKT Enzyme (Recombinant)

20-abx073794
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

UNG Enzyme (Recombinant)

20-abx073838
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

GAD1 Enzyme (Recombinant)

20-abx073843
  • EUR 393.60
  • EUR 7676.40
  • EUR 276.00
  • 10 ug
  • 1 mg
  • 2 µg

Recombinant Meiotic Recombination 11 Homolog A (MRE11A)

4-RPK113Hu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Meiotic Recombination 11 Homolog A expressed in: E.coli

Recombinant Bacillus Levanase Protein, His, Yeast-100ug

QP7478-ye-100ug 100ug
EUR 945.6

Recombinant Bacillus Levanase Protein, His, Yeast-10ug

QP7478-ye-10ug 10ug
EUR 434.4

Recombinant Bacillus Levanase Protein, His, Yeast-1mg

QP7478-ye-1mg 1mg
EUR 3296.4

Recombinant Bacillus Levanase Protein, His, Yeast-200ug

QP7478-ye-200ug 200ug
EUR 1512

Recombinant Bacillus Levanase Protein, His, Yeast-500ug

QP7478-ye-500ug 500ug
EUR 2164.8

Recombinant Bacillus Levanase Protein, His, Yeast-50ug

QP7478-ye-50ug 50ug
EUR 576

Recombinant Bacillus Anthracis Edema Factor (S447N Mutant)

VAng-Lsx1539-500g 500 µg
EUR 3198
Description: Bacillus Anthracis Edema Factor (S447N Mutant), recombinant protein.

Recombinant Bacillus Anthracis Lethal Factor (E687C Mutant)

VAng-Lsx1541-100g 100 µg
EUR 986.4
Description: Bacillus Anthracis Lethal Factor (E687C Mutant), recombinant protein.

Bacillus anthracis (Anthrax) antibody

10-B02A 1 mg
EUR 567.6
Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody

Bacillus anthracis (Anthrax) antibody

10-B02B 1 mg
EUR 562.8
Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody

Bacillus anthracis antibody (Spore)

10-B108A 500 ug
EUR 457.2
Description: Mouse monoclonal Bacillus anthracis antibody (Spore)

Bacillus anthracis antibody (Spore)

10-B108B 500 ug
EUR 457.2
Description: Mouse monoclonal Bacillus anthracis antibody (Spore)

Bacillus anthracis (Anthrax) antibody

20-BR04 500 ug
EUR 429.6
Description: Rabbit polyclonal Bacillus anthracis (Anthrax) antibody

Bacillus anthracis (Anthrax) antibody

10R-B106a 1 mg
EUR 681.6
Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody

Bacillus anthracis (Anthrax) antibody

10R-B106b 1 mg
EUR 681.6
Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody

Bacillus anthracis (Anthrax) antibody

10R-B106c 1 mg
EUR 567.6
Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody

Bacillus anthracis (Anthrax) antibody

10R-B106d 1 mg
EUR 567.6
Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody

Bacillus anthracis (Anthrax) antibody

10R-B106e 1 mg
EUR 562.8
Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody

Bacillus anthracis (Anthrax) antibody

10R-B106f 1 mg
EUR 681.6
Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody

Bacillus Circulans Intein Protein

20-abx261511
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Bacillus subtilis Levanase (sacC)

1-CSB-EP356581BRJ
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus subtilis Levanase(sacC) expressed in E.coli

Bacillus sp. Levanase (Levanase)

1-CSB-YP523248BRG
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus sp. Levanase(Levanase),partial expressed in Yeast

Native Bacillus licheniformis Protease

NATE-0633