VirB, a key transcriptional regulator of virulence plasmid genes in Shigella flexneri, varieties DNA-binding site-dependent foci within the bacterial cytoplasm
VirB is a key regulator of genes situated on the massive virulence plasmid (pINV) within the bacterial pathogen Shigella flexneri VirB is uncommon; it’s not associated to different transcriptional regulators, as an alternative, it belongs to a household of proteins that primarily operate in plasmid and chromosome partitioning; exemplified by ParB. Regardless of this, VirB doesn’t operate to segregate DNA, however moderately counters transcriptional silencing mediated by the nucleoid structuring protein, H-NS.
Since ParB localizes subcellularly as discrete foci within the bacterial cytoplasm, we selected to analyze the subcellular localization of VirB to realize novel perception into how VirB capabilities as a transcriptional anti-silencer.
To do that, a GFP-VirB fusion that retains the regulatory exercise of VirB and but, doesn’t bear important protein degradation in S. flexneri, was used. Surprisingly, discrete fluorescent foci had been noticed in reside wild-type S. flexneri cells and an isogenic virB mutant utilizing fluorescence microscopy.
In distinction, foci had been hardly ever noticed (<10%) in pINV-cured cells or in cells expressing a GFP-VirB fusion carrying amino acid substitutions within the VirB DNA binding area. Lastly, the 25 bp VirB-binding web site was demonstrated to be enough and vital for GFP-VirB focus formation utilizing a set of small surrogate plasmids.
Mixed, these information show that the VirB:DNA interactions required for the transcriptional anti-silencing exercise of VirB on pINV are a prerequisite for the subcellular localization of VirB within the bacterial cytoplasm. The importance of those findings, in gentle of the anti-silencing exercise of VirB, is mentioned.
SignificanceThis research reveals the subcellular localization of VirB, a key transcriptional regulator of virulence genes discovered on the massive virulence plasmid (pINV) in Shigella. Fluorescent alerts generated by an energetic GFP-VirB fusion type 2, 3, or Four discrete foci within the bacterial cytoplasm, predominantly on the quarter cell place. These alerts are fully dependent upon VirB interacting with its DNA binding web site discovered both on the virulence plasmid or an engineered surrogate.
Our findings:
1) present novel perception into VirB:pINV interactions,
2) counsel that VirB might have utility as a DNA marker, and
3) elevate questions on how and why this anti-silencing protein controls virulence gene expression on pINV of Shigella spp. varieties discrete foci/hubs inside the bacterial cytoplasm.
rmbiomed
Escherichia coli Outer membrane protein A (ompA) |
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| 1-CSB-CF364256ENV(A4) | Cusabio |
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Description: Recombinant Escherichia coli Outer membrane protein A(ompA) expressed in in vitro E.coli expression system |
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Escherichia coli F17 fimbrial protein (F17a-A) |
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| 1-CSB-EP318126ENL | Cusabio |
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Description: Recombinant Escherichia coli F17 fimbrial protein(F17a-A) expressed in E.coli |
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Escherichia coli Lipoate-protein ligase A (lplA) |
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| 1-CSB-EP510777ENU | Cusabio |
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Description: Recombinant Escherichia coli Lipoate-protein ligase A(lplA) expressed in E.coli |
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Escherichia coli (E. coli) Antibody |
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| abx411320-1ml | Abbexa | 1 ml | EUR 509 |
Escherichia coli (E. coli) Antibody |
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| abx415692-01mg | Abbexa | 0.1 mg | EUR 634 |
Escherichia coli (E. coli) Antibody |
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| abx415712-1ml | Abbexa | 1 ml | EUR 606 |
Escherichia coli Glyoxylate/hydroxypyruvate reductase A (ghrA) |
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| 1-CSB-EP482379ENM | Cusabio |
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Description: Recombinant Escherichia coli Glyoxylate/hydroxypyruvate reductase A(ghrA) expressed in E.coli |
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Escherichia coli Glyoxylate/hydroxypyruvate reductase A (ghrA) |
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| 1-CSB-EP482379ENMe1 | Cusabio |
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Description: Recombinant Escherichia coli Glyoxylate/hydroxypyruvate reductase A(ghrA) expressed in E.coli |
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Escherichia coli Protein (ECP) Protein |
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| 20-abx066464 | Abbexa |
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Escherichia coli J5 K88 Fimbrial Protein A (E. coli K88A / faeC) Antibody |
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| abx411325-02mg | Abbexa | 0.2 mg | EUR 565 |
Escherichia coli O157:H7 Outer membrane protein A (ompA) |
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| 1-CSB-EP359226EOD | Cusabio |
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Description: Recombinant Escherichia coli O157:H7 Outer membrane protein A(ompA),partial expressed in E.coli |
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Escherichia coli Type-1 fimbrial protein, A chain (fimA) |
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| 1-CSB-EP361210ENV | Cusabio |
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Description: Recombinant Escherichia coli Type-1 fimbrial protein, A chain(fimA) expressed in E.coli |
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Escherichia coli Heat-Labile Enterotoxin A (E. coli Labile Toxin A / eltA) Antibody |
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| abx411329-02mg | Abbexa | 0.2 mg | EUR 565 |
Escherichia coli Protein yebF (yebF) |
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| 1-CSB-EP329240ENV | Cusabio |
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Description: Recombinant Escherichia coli Protein yebF(yebF) expressed in E.coli |
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Escherichia Coli Protein (ECP) Antibody |
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| 20-abx102354 | Abbexa |
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Escherichia Coli Protein (ECP) Antibody |
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| 20-abx218321 | Abbexa |
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Escherichia coli Protein ELISA Kit |
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| ELA-E1655o | Lifescience Market | 96 Tests | EUR 928 |
Escherichia coli (E. coli) Antibody (Biotin) |
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| abx411321-1ml | Abbexa | 1 ml | EUR 592 |
Escherichia coli (E. coli) Antibody (FITC) |
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| abx411322-1ml | Abbexa | 1 ml | EUR 509 |
Escherichia coli (E. coli) Antibody (Biotin) |
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| abx415713-1ml | Abbexa | 1 ml | EUR 718 |
Escherichia coli Ribose-5-phosphate isomerase A (rpiA) |
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| 1-CSB-YP540419ENT | Cusabio |
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Description: Recombinant Escherichia coli Ribose-5-phosphate isomerase A(rpiA) expressed in Yeast |
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Escherichia coli Heat-labile enterotoxin A chain (eltA) |
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| 1-CSB-EP356922ENL | Cusabio |
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Description: Recombinant Escherichia coli Heat-labile enterotoxin A chain(eltA) expressed in E.coli |
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Escherichia coli Heat-labile enterotoxin A chain (eltA) |
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| 1-CSB-EP356922ENLa3 | Cusabio |
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Description: Recombinant Escherichia coli Heat-labile enterotoxin A chain(eltA) expressed in E.coli |
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Escherichia coli Ribose-5-phosphate isomerase A (rpiA) |
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| 1-CSB-EP540419ENT | Cusabio |
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Description: Recombinant Escherichia coli Ribose-5-phosphate isomerase A(rpiA) expressed in E.coli |
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Escherichia coli Flagellin (fliC) |
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| 1-CSB-EP300968ENV | Cusabio |
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Description: Recombinant Escherichia coli Flagellin(fliC) expressed in E.coli |
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Escherichia coli Flagellin (fliC) |
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| 1-CSB-EP300968ENVe1 | Cusabio |
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Description: Recombinant Escherichia coli Flagellin(fliC) expressed in E.coli |
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Escherichia coli Exopolyphosphatase (ppx) |
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| 1-CSB-EP365319ENV | Cusabio |
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Description: Recombinant Escherichia coli Exopolyphosphatase(ppx) expressed in E.coli |
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Escherichia coli Agmatinase (speB) |
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| 1-CSB-EP001447ENM | Cusabio |
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Description: Recombinant Escherichia coli Agmatinase(speB) expressed in E.coli |
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Escherichia coli Ribokinase (rbsK) |
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| 1-CSB-EP019397ENV | Cusabio |
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Description: Recombinant Escherichia coli Ribokinase(rbsK) expressed in E.coli |
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Escherichia coli PCR kit |
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| PCR-VHA207-48D | Bioingentech | 50T | EUR 453 |
Escherichia coli PCR kit |
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| PCR-VHA207-96D | Bioingentech | 100T | EUR 572 |
Top10F Escherichia coli Strains |
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| S0024 | Lifescience Market | 100 ul | EUR 365 |
TG1 Escherichia coli Strains |
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| S0026 | Lifescience Market | 100 ul | EUR 365 |
HB101 Escherichia coli Strains |
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| S0031 | Lifescience Market | 100 ul | EUR 365 |
DH10B Escherichia coli Strains |
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| S0032 | Lifescience Market | 100 ul | EUR 365 |
DH10Bac Escherichia coli Strains |
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| S0033 | Lifescience Market | 100 ul | EUR 365 |
Stbl2 Escherichia coli Strains |
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| S0034 | Lifescience Market | 100 ul | EUR 365 |
Stbl3 Escherichia coli Strains |
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| S0035 | Lifescience Market | 100 ul | EUR 365 |
BJ5183 Escherichia coli Strains |
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| S0036 | Lifescience Market | 100 ul | EUR 365 |
JM110 Escherichia coli Strains |
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| S0037 | Lifescience Market | 100 ul | EUR 365 |
ER2566 Escherichia coli Strains |
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| S0039 | Lifescience Market | 100 ul | EUR 365 |
SURE Escherichia coli Strains |
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| S0041 | Lifescience Market | 100 ul | EUR 365 |
DB3.1 Escherichia coli Strains |
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| S0043 | Lifescience Market | 100 ul | EUR 365 |
TransB Escherichia coli Strains |
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| S0045 | Lifescience Market | 100 ul | EUR 365 |
Trans110 Escherichia coli Strains |
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| S0046 | Lifescience Market | 100 ul | EUR 365 |
MC1061 Escherichia coli Strains |
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| S0047 | Lifescience Market | 100 ul | EUR 365 |
T1 Escherichia coli Strains |
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| S0048 | Lifescience Market | 100 ul | EUR 365 |
EPI300 Escherichia coli Strains |
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| S0050 | Lifescience Market | 100 ul | EUR 365 |
BW25113 Escherichia coli Strains |
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| S0051 | Lifescience Market | 100 ul | EUR 365 |
BL21 Escherichia coli Strains |
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| S0053 | Lifescience Market | 100 ul | EUR 365 |
EPI400 Escherichia coli Strains |
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| S0087 | Lifescience Market | 100 ul | EUR 365 |
K802 Escherichia coli Strains |
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| S0089 | Lifescience Market | 100 ul | EUR 365 |
JM108 Escherichia coli Strains |
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| S0105 | Lifescience Market | 100 ul | EUR 365 |
HB2151 Escherichia coli Strains |
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| S0122 | Lifescience Market | 100 ul | EUR 365 |
Recombinant Escherichia coli aaeA Protein (aa 1-310) |
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| VAng-Lsx02138-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) p-hydroxybenzoic acid efflux pump subunit AaeA, recombinant protein. |
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Recombinant Escherichia coli aaeX Protein (aa 1-67) |
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| VAng-Lsx02140-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli O1:K1 / APEC Protein AaeX, recombinant protein. |
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Recombinant Escherichia coli acpP Protein (aa 1-78) |
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| VAng-Lsx02150-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2718 |
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Description: Escherichia coli (strain K12) Acyl carrier protein, recombinant protein. |
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Recombinant Escherichia coli acpP Protein (aa 1-78) |
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| VAng-Lsx02150-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1343 |
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Description: Escherichia coli (strain K12) Acyl carrier protein, recombinant protein. |
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Recombinant Escherichia coli afuB Protein (aa 1-115) |
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| VAng-Lsx02154-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Putative ferric transport system permease protein fbpB, recombinant protein. |
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Recombinant Escherichia coli agaD Protein (aa 1-263) |
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| VAng-Lsx02156-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) N-acetylgalactosamine permease IID component, recombinant protein. |
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Recombinant Escherichia coli alaC Protein (aa 1-412) |
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| VAng-Lsx02159-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2113 |
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Description: Escherichia coli (strain K12) Glutamate-pyruvate aminotransferase AlaC, recombinant protein. |
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Recombinant Escherichia coli APECO1_3373 Protein (aa 1-223) |
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| VAng-Lsx02163-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli O1:K1 / APEC Putative protein-disulfide oxidoreductase (Ecok1_30590), recombinant protein. |
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Recombinant Escherichia coli appC Protein (aa 1-514) |
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| VAng-Lsx02164-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Cytochrome bd-II oxidase subunit 1, recombinant protein. |
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Recombinant Escherichia coli argO Protein (aa 1-211) |
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| VAng-Lsx02168-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Arginine exporter protein ArgO, recombinant protein. |
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Recombinant Escherichia coli arnC Protein (aa 1-322) |
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| VAng-Lsx02169-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase, recombinant protein. |
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Recombinant Escherichia coli codA Protein (aa 2-427) |
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| VAng-Lsx02222-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3294 |
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Description: Escherichia coli (strain K12) Cytosine deaminase, recombinant protein. |
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Recombinant Escherichia coli codA Protein (aa 2-427) |
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| VAng-Lsx02222-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 573 |
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Description: Escherichia coli (strain K12) Cytosine deaminase, recombinant protein. |
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Recombinant Escherichia coli col Protein (aa 1-180) |
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| VAng-Lsx02223-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2388 |
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Description: Escherichia coli Colicin-E5, recombinant protein. |
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Recombinant Escherichia coli col Protein (aa 1-180) |
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| VAng-Lsx02223-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1645 |
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Description: Escherichia coli Colicin-E5, recombinant protein. |
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Recombinant Escherichia coli col Protein (aa 1-205) |
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| VAng-Lsx02224-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3514 |
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Description: Escherichia coli Colicin-E8, recombinant protein. |
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Recombinant Escherichia coli col Protein (aa 1-205) |
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| VAng-Lsx02224-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2511 |
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Description: Escherichia coli Colicin-E8, recombinant protein. |
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Recombinant Escherichia coli col Protein (aa 1-581) |
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| VAng-Lsx02225-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5879 |
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Description: Escherichia coli Colicin-E2, recombinant protein. |
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Recombinant Escherichia coli col Protein (aa 1-582) |
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| VAng-Lsx02226-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5893 |
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Description: Escherichia coli Colicin-E9, recombinant protein. |
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Recombinant Escherichia coli col Protein (aa 1-582) |
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| VAng-Lsx02226-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4160 |
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Description: Escherichia coli Colicin-E9, recombinant protein. |
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Recombinant Escherichia coli col Protein (aa 1-582) |
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| VAng-Lsx02226-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2814 |
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Description: Escherichia coli Colicin-E9, recombinant protein. |
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Recombinant Escherichia coli colE7 Protein (aa 1-576) |
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| VAng-Lsx02227-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5852 |
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Description: Escherichia coli Colicin-E7, recombinant protein. |
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Recombinant Escherichia coli colE7 Protein (aa 1-576) |
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| VAng-Lsx02227-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4133 |
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Description: Escherichia coli Colicin-E7, recombinant protein. |
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Recombinant Escherichia coli colE7 Protein (aa 1-576) |
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| VAng-Lsx02227-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2785 |
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Description: Escherichia coli Colicin-E7, recombinant protein. |
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Recombinant Escherichia coli cptA Protein (aa 1-577) |
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| VAng-Lsx02236-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Phosphoethanolamine transferase CptA, recombinant protein. |
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Recombinant Escherichia coli creC Protein (aa 1-474) |
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| VAng-Lsx02250-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Sensor protein CreC, recombinant protein. |
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Recombinant Escherichia coli creD Protein (aa 1-450) |
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| VAng-Lsx02251-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Inner membrane protein CreD, recombinant protein. |
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Recombinant Escherichia coli csrD Protein (aa 1-646) |
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| VAng-Lsx02252-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) RNase E specificity factor CsrD, recombinant protein. |
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Recombinant Escherichia coli cssD Protein (aa 1-802) |
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| VAng-Lsx02253-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Outer membrane usher protein CssD (aa 1-802), recombinant protein. |
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Recombinant Escherichia coli cssD Protein (aa 1-819) |
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| VAng-Lsx02254-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Outer membrane usher protein CssD, recombinant protein. |
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Recombinant Escherichia coli cta Protein (aa 1-490) |
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| VAng-Lsx02255-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Colicin-10, recombinant protein. |
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Recombinant Escherichia coli cusS Protein (aa 1-480) |
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| VAng-Lsx02256-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Sensor kinase CusS, recombinant protein. |
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Recombinant Escherichia coli cvaA Protein (aa 1-413) |
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| VAng-Lsx02257-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5178 |
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Description: Escherichia coli Colicin V secretion protein CvaA, recombinant protein. |
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Recombinant Escherichia coli cvaA Protein (aa 1-413) |
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| VAng-Lsx02257-1mgYeast | Creative Biolabs | 1 mg (Yeast) | EUR 6429 |
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Description: Escherichia coli Colicin V secretion protein CvaA, recombinant protein. |
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Recombinant Escherichia coli cvaB Protein (aa 1-698) |
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| VAng-Lsx02258-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli Colicin V secretion/processing ATP-binding protein CvaB, recombinant protein. |
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Recombinant Escherichia coli cvaC Protein (aa 16-103) |
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| VAng-Lsx02259-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2773 |
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Description: Escherichia coli Colicin-V, recombinant protein. |
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Recombinant Escherichia coli cvaC Protein (aa 16-103) |
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| VAng-Lsx02259-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 1989 |
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Description: Escherichia coli Colicin-V, recombinant protein. |
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Recombinant Escherichia coli cvaC Protein (aa 16-103) |
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| VAng-Lsx02259-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1370 |
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Description: Escherichia coli Colicin-V, recombinant protein. |
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Recombinant Escherichia coli cvi Protein (aa 1-78) |
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| VAng-Lsx02260-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2718 |
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Description: Escherichia coli Colicin-V immunity protein, recombinant protein. |
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Recombinant Escherichia coli cvi Protein (aa 1-78) |
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| VAng-Lsx02260-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 1934 |
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Description: Escherichia coli Colicin-V immunity protein, recombinant protein. |
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Recombinant Escherichia coli cvi Protein (aa 1-78) |
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| VAng-Lsx02260-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1343 |
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Description: Escherichia coli Colicin-V immunity protein, recombinant protein. |
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Recombinant Escherichia coli cybB Protein (aa 1-176) |
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| VAng-Lsx02262-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Cytochrome b561, recombinant protein. |
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Recombinant Escherichia coli cydC Protein (aa 1-573) |
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| VAng-Lsx02263-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) ATP-binding/permease protein CydC, recombinant protein. |
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Recombinant Escherichia coli cydD Protein (aa 1-588) |
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| VAng-Lsx02264-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) ATP-binding/permease protein CydD, recombinant protein. |
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Recombinant Escherichia coli cyoA Protein (aa 25-315) |
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| VAng-Lsx02265-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Ubiquinol oxidase subunit 2, recombinant protein. |
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Recombinant Escherichia coli cyoC Protein (aa 1-204) |
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| VAng-Lsx02266-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Cytochrome o ubiquinol oxidase subunit 3, recombinant protein. |
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Recombinant Escherichia coli cyoD Protein (aa 1-109) |
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| VAng-Lsx02267-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Cytochrome o ubiquinol oxidase protein CyoD, recombinant protein. |
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Recombinant Escherichia coli cyoE Protein (aa 1-296) |
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| VAng-Lsx02268-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Protoheme IX farnesyltransferase, recombinant protein. |
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Recombinant Escherichia coli cysU Protein (aa 1-277) |
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| VAng-Lsx02269-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Sulfate transport system permease protein CysT, recombinant protein. |
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Recombinant Escherichia coli cysW Protein (aa 1-291) |
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| VAng-Lsx02270-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Sulfate transport system permease protein CysW, recombinant protein. |
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Recombinant Escherichia coli damX Protein (aa 1-428) |
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| VAng-Lsx02287-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Protein damX, recombinant protein. |
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Recombinant Escherichia coli dapB Protein (aa 1-273) |
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| VAng-Lsx02304-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3940 |
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Description: Escherichia coli O9:H4 Dihydrodipicolinate reductase, recombinant protein. |
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Recombinant Escherichia coli dapB Protein (aa 1-273) |
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| VAng-Lsx02304-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2785 |
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Description: Escherichia coli O9:H4 Dihydrodipicolinate reductase, recombinant protein. |
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Recombinant Escherichia coli dapB Protein (aa 1-273) |
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| VAng-Lsx02304-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1906 |
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Description: Escherichia coli O9:H4 Dihydrodipicolinate reductase, recombinant protein. |
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Recombinant Escherichia coli dapD Protein (aa 1-274) |
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| VAng-Lsx02321-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3940 |
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Description: Escherichia coli O157:H7 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, recombinant protein. |
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Recombinant Escherichia coli dapD Protein (aa 1-274) |
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| VAng-Lsx02321-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2800 |
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Description: Escherichia coli O157:H7 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, recombinant protein. |
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Recombinant Escherichia coli dapD Protein (aa 1-274) |
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| VAng-Lsx02321-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1906 |
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Description: Escherichia coli O157:H7 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, recombinant protein. |
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Recombinant Escherichia coli dcm Protein (aa 1-472) |
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| VAng-Lsx02337-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2566 |
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Description: Escherichia coli (strain K12) DNA-cytosine methyltransferase, recombinant protein. |
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Recombinant Escherichia coli ddpB Protein (aa 1-340) |
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| VAng-Lsx02338-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Probable D,D-dipeptide transport system permease protein ddpB, recombinant protein. |
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Recombinant Escherichia coli ddpC Protein (aa 1-298) |
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| VAng-Lsx02339-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli (strain K12) Probable D,D-dipeptide transport system permease protein ddpC, recombinant protein. |
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Recombinant Escherichia coli dedA Protein (aa 1-219) |
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| VAng-Lsx02341-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Escherichia coli O157:H7 Protein dedA, recombinant protein. |
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Recombinant Escherichia coli def Protein (aa 2-169) |
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| VAng-Lsx02342-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3268 |
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Description: Escherichia coli Peptide deformylase, recombinant protein. |
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Recombinant Escherichia coli def Protein (aa 2-169) |
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| VAng-Lsx02342-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2346 |
|
Description: Escherichia coli Peptide deformylase, recombinant protein. |
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Recombinant Escherichia coli def Protein (aa 2-169) |
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| VAng-Lsx02342-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1604 |
|
Description: Escherichia coli Peptide deformylase, recombinant protein. |
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Recombinant Escherichia coli degP Protein (aa 27-474) |
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| VAng-Lsx02343-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3294 |
|
Description: Escherichia coli Periplasmic serine endoprotease DegP, recombinant protein. |
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Recombinant Escherichia coli degP Protein (aa 27-474) |
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| VAng-Lsx02343-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2113 |
|
Description: Escherichia coli Periplasmic serine endoprotease DegP, recombinant protein. |
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Recombinant Escherichia coli degP Protein (aa 27-474) |
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| VAng-Lsx02343-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 573 |
|
Description: Escherichia coli Periplasmic serine endoprotease DegP, recombinant protein. |
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Recombinant Escherichia coli dpiB Protein (aa 1-552) |
|||
| VAng-Lsx02344-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Sensor histidine kinase DpiB, recombinant protein. |
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Recombinant Escherichia coli dppB Protein (aa 1-339) |
|||
| VAng-Lsx02346-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli O157:H7 Dipeptide transport system permease protein dppB, recombinant protein. |
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Recombinant Escherichia coli dppC Protein (aa 1-300) |
|||
| VAng-Lsx02349-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli O157:H7 Dipeptide transport system permease protein dppC, recombinant protein. |
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Recombinant Escherichia coli dsbB Protein (aa 1-176) |
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| VAng-Lsx02353-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli O1:K1 / APEC Disulfide bond formation protein B, recombinant protein. |
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Recombinant Escherichia coli eamB Protein (aa 1-195) |
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| VAng-Lsx02354-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Cysteine/O-acetylserine efflux protein, recombinant protein. |
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Recombinant Escherichia coli ecnA Protein (aa 19-41) |
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| VAng-Lsx02355-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2374 |
|
Description: Escherichia coli O157:H7 Entericidin A, recombinant protein. |
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Recombinant Escherichia coli ecnA Protein (aa 19-41) |
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| VAng-Lsx02355-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 1700 |
|
Description: Escherichia coli O157:H7 Entericidin A, recombinant protein. |
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Recombinant Escherichia coli ecnA Protein (aa 19-41) |
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| VAng-Lsx02355-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1178 |
|
Description: Escherichia coli O157:H7 Entericidin A, recombinant protein. |
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Recombinant Escherichia coli ecnB Protein (aa 22-48) |
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| VAng-Lsx02357-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2401 |
|
Description: Escherichia coli O157:H7 Entericidin B, recombinant protein. |
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Recombinant Escherichia coli ecnB Protein (aa 22-48) |
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| VAng-Lsx02357-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 1714 |
|
Description: Escherichia coli O157:H7 Entericidin B, recombinant protein. |
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Recombinant Escherichia coli ecnB Protein (aa 22-48) |
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| VAng-Lsx02357-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1191 |
|
Description: Escherichia coli O157:H7 Entericidin B, recombinant protein. |
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Recombinant Escherichia coli efeU Protein (aa 1-276) |
|||
| VAng-Lsx02359-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Putative inactive ferrous iron permease EfeU, recombinant protein. |
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Recombinant Escherichia coli efp Protein (aa 2-188) |
|||
| VAng-Lsx02360-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3679 |
|
Description: Escherichia coli (strain K12) Elongation factor P, recombinant protein. |
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Recombinant Escherichia coli efp Protein (aa 2-188) |
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| VAng-Lsx02360-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2374 |
|
Description: Escherichia coli (strain K12) Elongation factor P, recombinant protein. |
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Recombinant Escherichia coli efp Protein (aa 2-188) |
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| VAng-Lsx02360-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 614 |
|
Description: Escherichia coli (strain K12) Elongation factor P, recombinant protein. |
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Recombinant Escherichia coli emrE Protein (aa 1-110) |
|||
| VAng-Lsx02363-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Multidrug transporter emrE, recombinant protein. |
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Recombinant Escherichia coli envZ Protein (aa 1-450) |
|||
| VAng-Lsx02364-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Osmolarity sensor protein EnvZ, recombinant protein. |
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Recombinant Escherichia coli eptA Protein (aa 1-547) |
|||
| VAng-Lsx02365-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Phosphoethanolamine transferase eptA, recombinant protein. |
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Recombinant Escherichia coli eptB Protein (aa 1-563) |
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| VAng-Lsx02366-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Phosphoethanolamine transferase eptB, recombinant protein. |
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Recombinant Escherichia coli etk Protein (aa 1-726) |
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| VAng-Lsx02367-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Tyrosine-protein kinase etk, recombinant protein. |
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Recombinant Escherichia coli exbB Protein (aa 1-244) |
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| VAng-Lsx02369-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli O157:H7 Biopolymer transport protein exbB, recombinant protein. |
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Recombinant Escherichia coli fabB Protein (aa 1-406) |
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| VAng-Lsx02371-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3294 |
|
Description: Escherichia coli (strain K12) 3-oxoacyl-[acyl-carrier-protein] synthase 1, recombinant protein. |
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Recombinant Escherichia coli fabB Protein (aa 1-406) |
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| VAng-Lsx02371-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2113 |
|
Description: Escherichia coli (strain K12) 3-oxoacyl-[acyl-carrier-protein] synthase 1, recombinant protein. |
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Recombinant Escherichia coli fabB Protein (aa 1-406) |
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| VAng-Lsx02371-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 573 |
|
Description: Escherichia coli (strain K12) 3-oxoacyl-[acyl-carrier-protein] synthase 1, recombinant protein. |
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Recombinant Escherichia coli fabF Protein (aa 2-413) |
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| VAng-Lsx02372-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3294 |
|
Description: Escherichia coli (strain K12) 3-oxoacyl-[acyl-carrier-protein] synthase 2, recombinant protein. |
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Recombinant Escherichia coli fabF Protein (aa 2-413) |
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| VAng-Lsx02372-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2113 |
|
Description: Escherichia coli (strain K12) 3-oxoacyl-[acyl-carrier-protein] synthase 2, recombinant protein. |
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Recombinant Escherichia coli fabF Protein (aa 2-413) |
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| VAng-Lsx02372-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 573 |
|
Description: Escherichia coli (strain K12) 3-oxoacyl-[acyl-carrier-protein] synthase 2, recombinant protein. |
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Recombinant Escherichia coli fanC Protein (aa 23-181) |
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| VAng-Lsx02373-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3213 |
|
Description: Escherichia coli K99 fimbrial protein, recombinant protein. |
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Recombinant Escherichia coli fanC Protein (aa 23-181) |
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| VAng-Lsx02373-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2305 |
|
Description: Escherichia coli K99 fimbrial protein, recombinant protein. |
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Recombinant Escherichia coli fanC Protein (aa 23-181) |
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| VAng-Lsx02373-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1576 |
|
Description: Escherichia coli K99 fimbrial protein, recombinant protein. |
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Recombinant Escherichia coli fepE Protein (aa 1-377) |
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| VAng-Lsx02374-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Ferric enterobactin transport protein fepE, recombinant protein. |
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Recombinant Escherichia coli FimH Protein (aa 22-300) |
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| VAng-Lsx02377-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4793 |
|
Description: Escherichia coli protein FimH, recombinant protein. |
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Recombinant Escherichia coli FimH Protein (aa 22-300) |
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| VAng-Lsx02377-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3048 |
|
Description: Escherichia coli protein FimH, recombinant protein. |
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Recombinant Escherichia coli FimH Protein (aa 22-300) |
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| VAng-Lsx02377-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 903 |
|
Description: Escherichia coli protein FimH, recombinant protein. |
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Recombinant Escherichia coli flhB Protein (aa 1-382) |
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| VAng-Lsx02378-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Flagellar biosynthetic protein flhB, recombinant protein. |
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Recombinant Escherichia coli fliF Protein (aa 1-552) |
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| VAng-Lsx02379-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Flagellar M-ring protein, recombinant protein. |
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Recombinant Escherichia coli fliL Protein (aa 1-154) |
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| VAng-Lsx02381-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli O157:H7 Flagellar protein FliL, recombinant protein. |
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Recombinant Escherichia coli fliO Protein (aa 1-121) |
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| VAng-Lsx02383-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Flagellar protein fliO, recombinant protein. |
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Recombinant Escherichia coli fliP Protein (aa 22-245) |
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| VAng-Lsx02384-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli Flagellar biosynthetic protein fliP, recombinant protein. |
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Recombinant Escherichia coli fliQ Protein (aa 1-89) |
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| VAng-Lsx02387-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli O157:H7 Flagellar biosynthetic protein FliQ, recombinant protein. |
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Recombinant Escherichia coli focA Protein (aa 1-285) |
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| VAng-Lsx02390-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli O157:H7 Probable formate transporter 1, recombinant protein. |
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Recombinant Escherichia coli focB Protein (aa 1-282) |
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| VAng-Lsx02391-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Probable formate transporter 2, recombinant protein. |
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Recombinant Escherichia coli frdC Protein (aa 1-131) |
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| VAng-Lsx02392-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Fumarate reductase subunit C, recombinant protein. |
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Recombinant Escherichia coli frdD Protein (aa 1-119) |
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| VAng-Lsx02393-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Fumarate reductase subunit D, recombinant protein. |
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Simplified plasmid cloning with a common MCS design and bacterial in vivo meeting
Background: The power to clone DNA sequences shortly and exactly into plasmids is important for molecular biology research. The latest improvement of seamless cloning applied sciences has made important enhancements in plasmid development, however easy and dependable instruments are at all times fascinating for time- and labor-saving functions.
Outcomes: We developed and standardized a plasmid cloning protocol primarily based on a common MCS (A number of Cloning Web site) design and bacterial in vivo meeting. With this technique, the vector is linearized first by PCR (Polymerase Chain Response) or restriction digestion.
Then a small quantity (10 ~ 20 ng) of this linear vector could be blended with a PCR-amplified insert (5× molar ratio towards vector) and remodeled straight into competent E. coli cells to acquire the specified clones by means of in vivo meeting.
Since we used a 36-bp common MCS because the homologous linker, any PCR-amplified insert with ~ 15 bp appropriate termini could be cloned into the vector with excessive constancy and effectivity.
Thus, the necessity for redesigning insert-amplifying primers in keeping with varied vector sequences and the next PCR procedures was eradicated.
Conclusions: Our protocol considerably diminished hands-on time for making ready transformation reactions, had wonderful reliability, and was confirmed to be a fast and versatile plasmid cloning approach. The protocol incorporates principally mixing steps, making it an especially automation-friendly and promising software in trendy biology research.
Key phrases: Bacterial in vivo meeting; DNA meeting; Homologous sequence; Plasmid cloning; Common MCS.
A ProQ/FinO household protein concerned in plasmid copy quantity management favours health of micro organism carrying mcr-1-bearing IncI2 plasmids
The plasmid-encoded colistin resistance gene mcr-1 challenges the usage of polymyxins and poses a menace to public well being. Though IncI2-type plasmids are the commonest vector for spreading the mcr-1 gene, the mechanisms by which these plasmids adapt to host micro organism and preserve resistance genes stay unclear.
- Herein, we investigated the regulatory mechanism for controlling the health value of an IncI2 plasmid carrying mcr-1.
- A putative ProQ/FinO household protein encoded by the IncI2 plasmid, designated as PcnR (plasmid copy quantity repressor), balances the mcr-1 expression and micro organism health by repressing the plasmid copy quantity.
- It binds to the primary stem-loop construction of the repR mRNA to repress RepA expression, which differs from some other beforehand reported plasmid replication management mechanism.
- Plasmid invasion experiments revealed that pcnR is important for the persistence of the mcr-1-bearing IncI2 plasmid within the bacterial populations. Moreover, single-copy mcr-1 gene nonetheless exerted a health value to host micro organism, and negatively affected the persistence of the IncI2 plasmid in aggressive co-cultures.
- These findings show that sustaining mcr-1 plasmid at a single copy is important for its persistence, and clarify the considerably diminished prevalence of mcr-1 following the ban of colistin as a progress promoter in China.
Bacillus thuringiensis,BT ELISA Kit |
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| CN-00140O1 | ChemNorm | 96T | EUR 473 |
Bacillus thuringiensis,BT ELISA Kit |
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| CN-00140O2 | ChemNorm | 48T | EUR 323 |
Bacillus thuringiensis subsp. Pesticidal crystal protein cry1Ac (cry1Ac) |
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| 1-CSB-YP356448BDC | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp. Pesticidal crystal protein cry1Ac(cry1Ac),partial expressed in Yeast |
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Bacillus thuringiensis subsp. Pesticidal crystal protein cry3Aa (cry3Aa) |
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| 1-CSB-YP358337BDG | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp. Pesticidal crystal protein cry3Aa(cry3Aa) expressed in Yeast |
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Bacillus thuringiensis subsp. Pesticidal crystal protein cry1Ab (cry1Ab) |
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| 1-CSB-YP363358BDC | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp. Pesticidal crystal protein cry1Ab(cry1Ab),partial expressed in Yeast |
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Bacillus thuringiensis subsp.Kurstaki Pesticidal crystal protein cry2Ab (cry2Ab) |
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| 1-CSB-EP324157BDC | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp.Kurstaki Pesticidal crystal protein cry2Ab(cry2Ab) expressed in E.coli |
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Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac (cry1Ac) |
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| 1-CSB-EP356448BDC | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac(cry1Ac),partial expressed in E.coli |
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Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa (cry3Aa) |
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| 1-CSB-EP358337BDG | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa(cry3Aa) expressed in E.coli |
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Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa (cry3Aa) |
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| 1-CSB-EP358337BDGa1 | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp. tenebrionis Pesticidal crystal protein cry3Aa(cry3Aa) expressed in E.coli |
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Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ab (cry1Ab) |
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| 1-CSB-EP363358BDC | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ab(cry1Ab),partial expressed in E.coli |
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Bacillus thuringiensis subsp. morrisoni Pesticidal crystal protein cry1Fb (cry1Fb) |
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| 1-CSB-EP528987BRS | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp. morrisoni Pesticidal crystal protein cry1Fb(cry1Fb),partial expressed in E.coli |
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Bacillus thuringiensis subsp. morrisoni Pesticidal crystal protein Cry1Fb (cry1Fb) |
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| 1-CSB-EP528987BRSa0 | Cusabio |
|
|
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Description: Recombinant Bacillus thuringiensis subsp. morrisoni Pesticidal crystal protein Cry1Fb(cry1Fb),partial expressed in E.coli |
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Recombinant Bacillus Thermolysin Protein |
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| VAng-Lsx1547-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Bacillus Thermolysin, recombinant protein from Bacillus. |
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PFU enzyme Plasmid |
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| PVT7002 | Lifescience Market | 2 ug | EUR 512 |
Recombinant Bacillus Anthracis Protective Proein |
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| VAng-Lsx1537-50g | Creative Biolabs | 50 µg | EUR 536 |
|
Description: Bacillus Anthracis Protective Protein, recombinant protein from P. fluorescens. |
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Recombinant Bacillus Anthracis Edema Factor |
|||
| VAng-Lsx1538-100g | Creative Biolabs | 100 µg | EUR 448 |
|
Description: Bacillus Anthracis Edema Factor, recombinant protein. |
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Recombinant Bacillus Anthracis Lethal Factor |
|||
| VAng-Lsx1540-1mg | Creative Biolabs | 1 mg | EUR 1975 |
|
Description: Bacillus Anthracis Lethal Factor, recombinant protein. |
|||
Recombinant Bacillus Thermoglucosidasius groL Protein |
|||
| VAng-Lsx1544-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Bacillus thermoglucosidasius 60 kDa chaperonin], recombinant protein. |
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Recombinant Bacillus Thermoglucosidasius dnaK Protein |
|||
| VAng-Lsx1545-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Bacillus thermoglucosidasius Chaperone protein DnaK, recombinant protein. |
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Recombinant Bacillus Circulans Intein Protein [His] |
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| VAng-Lsx1542-100g | Creative Biolabs | 100 µg | EUR 904 |
|
Description: Bacillus Circulans Intein [His], recombinant protein. |
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Recombinant Bacillus Circulans Intein Protein [His] |
|||
| VAng-Lsx1542-500g | Creative Biolabs | 500 µg | EUR 2027 |
|
Description: Bacillus Circulans Intein [His], recombinant protein. |
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Glycine Oxidase H244K, Bacillus subtilis recombinant |
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| 7845-1000 | Biovision | EUR 1784 | |
Glycine Oxidase H244K, Bacillus subtilis recombinant |
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| 7845-250 | Biovision | EUR 588 | |
Bacillus cereus Enterotoxin |
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| 1-CSB-EP302168BQJ | Cusabio |
|
|
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Description: Recombinant Bacillus cereus Enterotoxin,partial expressed in E.coli |
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Bacillus amyloliquefaciens Barstar |
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| 1-CSB-EP320008BQB | Cusabio |
|
|
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Description: Recombinant Bacillus amyloliquefaciens Barstar expressed in E.coli |
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Bacillus sp. Levanase |
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| 1-CSB-EP523248BRG | Cusabio |
|
|
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Description: Recombinant Bacillus sp. Levanase,partial expressed in E.coli |
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Bacillus Spores Antibody |
|||
| abx022927-1ml | Abbexa | 1 ml | EUR 599 |
BACILLUS CEREUS AGAR |
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| B02-101-10kg | Alphabiosciences | 10 kg | EUR 1823 |
BACILLUS CEREUS AGAR |
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| B02-101-2Kg | Alphabiosciences | 2 Kg | EUR 436 |
BACILLUS CEREUS AGAR |
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| B02-101-500g | Alphabiosciences | 500 g | EUR 155 |
Recombinant tTG Enzyme |
|||
| abx070024-100ug | Abbexa | 100 ug | EUR 439 |
HRASLS3 Enzyme (Recombinant) |
|||
| 20-abx073048 | Abbexa |
|
|
AHCY Enzyme (Recombinant) |
|||
| 20-abx073135 | Abbexa |
|
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HTATIP2 Enzyme (Recombinant) |
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| 20-abx073312 | Abbexa |
|
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ADPRHL2 Enzyme (Recombinant) |
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| 20-abx073359 | Abbexa |
|
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ARSG Enzyme (Recombinant) |
|||
| 20-abx073379 | Abbexa |
|
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ARSA Enzyme (Recombinant) |
|||
| 20-abx073395 | Abbexa |
|
|
B3GAT3 Enzyme (Recombinant) |
|||
| 20-abx073397 | Abbexa |
|
|
GLUD1 Enzyme (Recombinant) |
|||
| 20-abx073437 | Abbexa |
|
|
TPO Enzyme (Recombinant) |
|||
| 20-abx073668 | Abbexa |
|
|
ADA Enzyme (Recombinant) |
|||
| 20-abx073712 | Abbexa |
|
|
TKT Enzyme (Recombinant) |
|||
| 20-abx073794 | Abbexa |
|
|
UNG Enzyme (Recombinant) |
|||
| 20-abx073838 | Abbexa |
|
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GAD1 Enzyme (Recombinant) |
|||
| 20-abx073843 | Abbexa |
|
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pET28a- ULP (SUMO enzyme) Plasmid |
|||
| PVT7015 | Lifescience Market | 2 ug | EUR 512 |
Recombinant Bacillus Anthracis Edema Factor (S447N Mutant) |
|||
| VAng-Lsx1539-500g | Creative Biolabs | 500 µg | EUR 2665 |
|
Description: Bacillus Anthracis Edema Factor (S447N Mutant), recombinant protein. |
|||
Recombinant Bacillus Anthracis Lethal Factor (E687C Mutant) |
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| VAng-Lsx1541-100g | Creative Biolabs | 100 µg | EUR 822 |
|
Description: Bacillus Anthracis Lethal Factor (E687C Mutant), recombinant protein. |
|||
Recombinant Bacillus Levanase Protein, His, Yeast-100ug |
|||
| QP7478-ye-100ug | EnQuireBio | 100ug | EUR 788 |
Recombinant Bacillus Levanase Protein, His, Yeast-10ug |
|||
| QP7478-ye-10ug | EnQuireBio | 10ug | EUR 362 |
Recombinant Bacillus Levanase Protein, His, Yeast-1mg |
|||
| QP7478-ye-1mg | EnQuireBio | 1mg | EUR 2747 |
Recombinant Bacillus Levanase Protein, His, Yeast-200ug |
|||
| QP7478-ye-200ug | EnQuireBio | 200ug | EUR 1260 |
Recombinant Bacillus Levanase Protein, His, Yeast-500ug |
|||
| QP7478-ye-500ug | EnQuireBio | 500ug | EUR 1804 |
Recombinant Bacillus Levanase Protein, His, Yeast-50ug |
|||
| QP7478-ye-50ug | EnQuireBio | 50ug | EUR 480 |
Recombinant Meiotic Recombination 11 Homolog A (MRE11A) |
|||
| 4-RPK113Hu01 | Cloud-Clone |
|
|
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Description: Recombinant Human Meiotic Recombination 11 Homolog A expressed in: E.coli |
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Bacillus sp. Levanase (Levanase) |
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| 1-CSB-YP523248BRG | Cusabio |
|
|
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Description: Recombinant Bacillus sp. Levanase(Levanase),partial expressed in Yeast |
|||
Bacillus subtilis Levanase (sacC) |
|||
| 1-CSB-EP356581BRJ | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Levanase(sacC) expressed in E.coli |
|||
Native Bacillus licheniformis Protease |
|||
| NATE-0633 | Creative Enzymes | 100mg | EUR 411 |
Bacillus cereus PCR kit |
|||
| PCR-H492-48D | Bioingentech | 50T | EUR 453 |
Bacillus cereus PCR kit |
|||
| PCR-H492-96D | Bioingentech | 100T | EUR 572 |
Bacillus anthracis PCR kit |
|||
| PCR-H504-48D | Bioingentech | 50T | EUR 453 |
Bacillus anthracis PCR kit |
|||
| PCR-H504-96D | Bioingentech | 100T | EUR 572 |
Bacillus Circulans Intein Protein |
|||
| 20-abx261511 | Abbexa |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106a | Fitzgerald | 1 mg | EUR 568 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106b | Fitzgerald | 1 mg | EUR 568 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106c | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106d | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106e | Fitzgerald | 1 mg | EUR 469 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10R-B106f | Fitzgerald | 1 mg | EUR 568 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 20-BR04 | Fitzgerald | 500 ug | EUR 358 |
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Description: Rabbit polyclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10-B02A | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis (Anthrax) antibody |
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| 10-B02B | Fitzgerald | 1 mg | EUR 469 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody |
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Bacillus anthracis antibody (Spore) |
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| 10-B108A | Fitzgerald | 500 ug | EUR 381 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Spore) |
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Bacillus anthracis antibody (Spore) |
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| 10-B108B | Fitzgerald | 500 ug | EUR 381 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Spore) |
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BS168 Bacillus subtilis Strains |
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| S0075 | Lifescience Market | 100 ul | EUR 365 |
WB800N Bacillus subtilis Strains |
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| S0076 | Lifescience Market | 100 ul | EUR 365 |
SCK6 Bacillus subtilis Strains |
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| S0086 | Lifescience Market | 100 ul | EUR 365 |
WB600 Bacillus subtilis Strains |
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| S0093 | Lifescience Market | 100 ul | EUR 365 |
Streptolysin O Enzyme (Recombinant) |
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| abx070023-1mg | Abbexa | 1 mg | EUR 328 |
Heparanase 1 Enzyme (Recombinant) |
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| abx073026-100ng | Abbexa | 100 ng | EUR 704 |
Heparanase 1 Enzyme (Recombinant) |
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| abx073026-200ng | Abbexa | 200 ng | EUR 1247 |
Heparanase 1 Enzyme (Recombinant) |
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| abx073026-300ng | Abbexa | 300 ng | EUR 1790 |
Malic Enzyme 2 (Recombinant) |
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| 20-abx073149 | Abbexa |
|
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Decapping Enzyme, Scavenger (Recombinant) |
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| 20-abx073718 | Abbexa |
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Insulin-Degrading Enzyme (Recombinant) |
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| 20-abx073853 | Abbexa |
|
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Recombinant Bacillus Intein Protein (aa 3-518) [His] |
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| VAng-Lsx1543-500g | Creative Biolabs | 500 µg | EUR 2250 |
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Description: Bacillus Intein (aa 3-518) [His], recombinant protein. |
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Recombinant Bacillus Levanase Protein, His-SUMO, E.coli-100ug |
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| QP7478-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant Bacillus Levanase Protein, His-SUMO, E.coli-10ug |
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| QP7478-ec-10ug | EnQuireBio | 10ug | EUR 326 |
Recombinant Bacillus Levanase Protein, His-SUMO, E.coli-1mg |
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| QP7478-ec-1mg | EnQuireBio | 1mg | EUR 2303 |
Recombinant Bacillus Levanase Protein, His-SUMO, E.coli-200ug |
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| QP7478-ec-200ug | EnQuireBio | 200ug | EUR 1115 |
Recombinant Bacillus Levanase Protein, His-SUMO, E.coli-500ug |
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| QP7478-ec-500ug | EnQuireBio | 500ug | EUR 1514 |
Recombinant Bacillus Levanase Protein, His-SUMO, E.coli-50ug |
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| QP7478-ec-50ug | EnQuireBio | 50ug | EUR 435 |
Bacillus subtilis Expansin-yoaJ (yoaJ) |
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| 1-CSB-YP522026BRJ | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Expansin-yoaJ(yoaJ) expressed in Yeast |
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Bacillus subtilis Alpha-galactosidase (melA) |
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| 1-CSB-YP523525BRJ | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Alpha-galactosidase(melA) expressed in Yeast |
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Bacillus subtilis Subtilisin E (aprE) |
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| 1-CSB-EP356214BRJ | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Subtilisin E(aprE) expressed in E.coli |
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Bacillus licheniformis Subtilisin Carlsberg (apr) |
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| 1-CSB-EP365470BQT | Cusabio |
|
|
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Description: Recombinant Bacillus licheniformis Subtilisin Carlsberg(apr) expressed in E.coli |
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Bacillus cereus Methylglyoxal synthase (mgsA) |
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| 1-CSB-EP507015BQK | Cusabio |
|
|
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Description: Recombinant Bacillus cereus Methylglyoxal synthase(mgsA) expressed in E.coli |
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Bacillus subtilis Glycine oxidase (thiO) |
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| 1-CSB-EP521738BRJ | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Glycine oxidase(thiO) expressed in E.coli |
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Bacillus subtilis Expansin-yoaJ (yoaJ) |
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| 1-CSB-EP522026BRJ | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Expansin-yoaJ(yoaJ) expressed in E.coli |
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Bacillus subtilis Subtilosin-A (sboA) |
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| 1-CSB-EP522484BRJ | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Subtilosin-A(sboA) expressed in E.coli |
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Bacillus subtilis Subtilosin-A (sboA) |
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| 1-CSB-EP522484BRJe3 | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Subtilosin-A(sboA) expressed in E.coli |
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Bacillus subtilis Alpha-galactosidase (melA) |
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| 1-CSB-EP523525BRJ | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Alpha-galactosidase(melA) expressed in E.coli |
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Bacillus anthracis Thymidylate kinase (tmk) |
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| 1-CSB-EP007226BQG | Cusabio |
|
|
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Description: Recombinant Bacillus anthracis Thymidylate kinase(tmk) expressed in E.coli |
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Bacillus subtilis Malate dehydrogenase (mdh) |
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| 1-CSB-EP013623BRJ | Cusabio |
|
|
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Description: Recombinant Bacillus subtilis Malate dehydrogenase(mdh) expressed in E.coli |
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Bacillus cereus RT PCR kit |
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| RTq-H492-100D | Bioingentech | 100T | EUR 717 |
Bacillus cereus RT PCR kit |
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| RTq-H492-150D | Bioingentech | 150T | EUR 808 |
Bacillus cereus RT PCR kit |
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| RTq-H492-50D | Bioingentech | 50T | EUR 598 |
Bacillus anthracis RT PCR kit |
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| RTq-H504-100D | Bioingentech | 100T | EUR 717 |
Bacillus anthracis RT PCR kit |
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| RTq-H504-150D | Bioingentech | 150T | EUR 808 |
Bacillus anthracis RT PCR kit |
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| RTq-H504-50D | Bioingentech | 50T | EUR 598 |
Cilia-associated bacillus PCR kit |
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| PCR-V343-48D | Bioingentech | 50T | EUR 453 |
Cilia-associated bacillus PCR kit |
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| PCR-V343-96D | Bioingentech | 100T | EUR 572 |
Native Bacillus subtilis Bilirubin oxidase |
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| DIA-129 | Creative Enzymes | 1KU | EUR 335 |
Native Bacillus megaterium Diaphorase (NADH) |
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| DIA-142 | Creative Enzymes | 10ku | EUR 335 |
Native Bacillus sp. Leucine dehydrogenase |
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| DIA-209 | Creative Enzymes | 5KU | EUR 2765 |
Neutral proteinase from Bacillus sp. |
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| DIA-273 | Creative Enzymes | 1g | EUR 270 |
alpha Amylase from Bacillus licheniformis |
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| 20-abx082217 | Abbexa |
|
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Bacillus anthracis (Anthrax) antibody (Spore) |
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| 10R-B108a | Fitzgerald | 1 mg | EUR 651 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Spore) |
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Bacillus anthracis (Anthrax) antibody (Spore) |
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| 10R-B108b | Fitzgerald | 1 mg | EUR 651 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Spore) |
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Bacillus anthracis antibody (Protective Antigen) |
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| 70-1007 | Fitzgerald | 1 mg | EUR 367 |
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Description: Polyclonal Bacillus anthracis antibody (Protective Antigen), Anti-Bacillus anthracis antibody (Protective Antigen), Bacillus anthracis PA antibody, Anthrax PA antibody, Bacillus anthracis Protective Antigen antibody, Anthrax Protective Antigen antibody |
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Bacillus anthracis antibody (Lethal Factor) |
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| 70-1008 | Fitzgerald | 1 mg | EUR 367 |
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Description: Polyclonal Bacillus anthracis antibody (Lethal Factor), Anti-Bacillus anthracis antibody (Lethal Factor), Bacillus anthracis LF antibody, Anthrax LF antibody, Bacillus anthracis Lethal Factor antibody, Anthrax Lethal Factor antibody |
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Bacillus anthracis antibody (Edema Factor) |
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| 70-1009 | Fitzgerald | 1 mg | EUR 438 |
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Description: Polyclonal Bacillus anthracis antibody (Edema Factor), Anti-Bacillus anthracis antibody (Edema Factor), Bacillus anthracis EF antibody, Anthrax EF antibody, Bacillus anthracis Edema Factor antibody, Anthrax Edema Factor antibody |
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Bacillus anthracis antibody (Lethal Factor) |
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| 10-7841 | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Lethal Factor) |
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Bacillus anthracis antibody (Lethal Factor) |
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| 10-7842 | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Lethal Factor) |
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Bacillus anthracis antibody (Lethal Factor) |
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| 10-B107A | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Lethal Factor) |
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Bacillus anthracis antibody (Lethal Factor) |
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| 10-B107B | Fitzgerald | 1 mg | EUR 473 |
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Description: Mouse monoclonal Bacillus anthracis antibody (Lethal Factor) |
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Bacillus anthracis (Anthrax) antibody (Spore) |
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| 10C-CR9004M3 | Fitzgerald | 100 ug | EUR 503 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Spore) |
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Bacillus anthracis (Anthrax) antibody (Spore) |
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| 10C-CR9004M4 | Fitzgerald | 100 ug | EUR 503 |
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Description: Mouse monoclonal Bacillus anthracis (Anthrax) antibody (Spore) |
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Recombination Protein RecR (RECR) Antibody |
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| 20-abx300706 | Abbexa |
|
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Recombination Protein UvsY (uvsY) Antibody |
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| 20-abx300851 | Abbexa |
|
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Recombinant Bacillus Thermoglucosidasius L-aminoacylase Protein (aa 1-24) |
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| VAng-Lsx1546-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 2577 |
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Description: Bacillus thermoglucosidasius N-acyl-L-amino acid amidohydrolase (aa 1-24), recombinant protein. |
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Recombinant Bacillus Thermoglucosidasius L-aminoacylase Protein (aa 1-24) |
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| VAng-Lsx1546-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 1747 |
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Description: Bacillus thermoglucosidasius N-acyl-L-amino acid amidohydrolase (aa 1-24), recombinant protein. |
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Recombinant Bacillus Thermoglucosidasius L-aminoacylase Protein (aa 1-24) |
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| VAng-Lsx1546-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1197 |
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Description: Bacillus thermoglucosidasius N-acyl-L-amino acid amidohydrolase (aa 1-24), recombinant protein. |
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Recombinant other Intein Bacillus Circulans Protein, His, E.coli-1mg |
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| QP12451-1mg | EnQuireBio | 1mg | EUR 2757 |
Recombinant other Intein Bacillus Circulans Protein, His, E.coli-20ug |
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| QP12451-20ug | EnQuireBio | 20ug | EUR 201 |
Recombinant other Intein Bacillus Circulans Protein, His, E.coli-5ug |
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| QP12451-5ug | EnQuireBio | 5ug | EUR 155 |
Recombinant Bacillus Monomeric sarcosine oxidase Protein, His, E.coli-100ug |
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| QP7152-ec-100ug | EnQuireBio | 100ug | EUR 707 |
Recombinant Bacillus Monomeric sarcosine oxidase Protein, His, E.coli-10ug |
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| QP7152-ec-10ug | EnQuireBio | 10ug | EUR 326 |
Recombinant Bacillus Monomeric sarcosine oxidase Protein, His, E.coli-1mg |
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| QP7152-ec-1mg | EnQuireBio | 1mg | EUR 2303 |
Recombinant Bacillus Monomeric sarcosine oxidase Protein, His, E.coli-200ug |
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| QP7152-ec-200ug | EnQuireBio | 200ug | EUR 1115 |
Recombinant Bacillus Monomeric sarcosine oxidase Protein, His, E.coli-500ug |
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| QP7152-ec-500ug | EnQuireBio | 500ug | EUR 1514 |
Recombinant Bacillus Monomeric sarcosine oxidase Protein, His, E.coli-50ug |
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| QP7152-ec-50ug | EnQuireBio | 50ug | EUR 435 |
Recombinant Insulin Degrading Enzyme (IDE) |
|||
| 4-RPB897Hu01 | Cloud-Clone |
|
|
|
Description: Recombinant Human Insulin Degrading Enzyme expressed in: E.coli |
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Recombinant Glycogen Debranching Enzyme (AGL) |
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| 4-RPD189Hu01 | Cloud-Clone |
|
|
|
Description: Recombinant Human Glycogen Debranching Enzyme expressed in: E.coli |
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Ubiquitin-Conjugating Enzyme E2I (Recombinant) |
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| 20-abx073049 | Abbexa |
|
|
Ubiquitin Conjugating Enzyme E2S (Recombinant) |
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| 20-abx073066 | Abbexa |
|
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Ubiquitin Conjugating Enzyme E2T (Recombinant) |
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| 20-abx073118 | Abbexa |
|
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Ubiquitin Conjugating Enzyme E2A (Recombinant) |
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| 20-abx073122 | Abbexa |
|
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Ubiquitin Conjugating Enzyme E2N (Recombinant) |
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| 20-abx073205 | Abbexa |
|
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Ubiquitin Conjugating Enzyme E2D1 (Recombinant) |
|||
| 20-abx073207 | Abbexa |
|
|
Ubiquitin Conjugating Enzyme E2W (Recombinant) |
|||
| 20-abx073208 | Abbexa |
|
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Ubiquitin Conjugating Enzyme E2D2 (Recombinant) |
|||
| 20-abx073231 | Abbexa |
|
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Ubiquitin Conjugating Enzyme E2D4 (Recombinant) |
|||
| 20-abx073246 | Abbexa |
|
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Ubiquitin Conjugating Enzyme E2M (Recombinant) |
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| 20-abx073285 | Abbexa |
|
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Ubiquitin Conjugating Enzyme E2C (Recombinant) |
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| 20-abx073286 | Abbexa |
|
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Ubiquitin-Conjugating Enzyme E2H (Recombinant) |
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| 20-abx073341 | Abbexa |
|
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Ubiquitin-Conjugating Enzyme E2F (Recombinant) |
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| 20-abx073351 | |||