VirB, a key transcriptional regulator of virulence plasmid genes in Shigella flexneri, varieties DNA-binding site-dependent foci within the bacterial cytoplasm
VirB is a key regulator of genes situated on the massive virulence plasmid (pINV) within the bacterial pathogen Shigella flexneri VirB is uncommon; it’s not associated to different transcriptional regulators, as an alternative, it belongs to a household of proteins that primarily operate in plasmid and chromosome partitioning; exemplified by ParB. Regardless of this, VirB doesn’t operate to segregate DNA, however moderately counters transcriptional silencing mediated by the nucleoid structuring protein, H-NS.
Since ParB localizes subcellularly as discrete foci within the bacterial cytoplasm, we selected to analyze the subcellular localization of VirB to realize novel perception into how VirB capabilities as a transcriptional anti-silencer.
To do that, a GFP-VirB fusion that retains the regulatory exercise of VirB and but, doesn’t bear important protein degradation in S. flexneri, was used. Surprisingly, discrete fluorescent foci had been noticed in reside wild-type S. flexneri cells and an isogenic virB mutant utilizing fluorescence microscopy.
In distinction, foci had been hardly ever noticed (<10%) in pINV-cured cells or in cells expressing a GFP-VirB fusion carrying amino acid substitutions within the VirB DNA binding area. Lastly, the 25 bp VirB-binding web site was demonstrated to be enough and vital for GFP-VirB focus formation utilizing a set of small surrogate plasmids.
Mixed, these information show that the VirB:DNA interactions required for the transcriptional anti-silencing exercise of VirB on pINV are a prerequisite for the subcellular localization of VirB within the bacterial cytoplasm. The importance of those findings, in gentle of the anti-silencing exercise of VirB, is mentioned.
SignificanceThis research reveals the subcellular localization of VirB, a key transcriptional regulator of virulence genes discovered on the massive virulence plasmid (pINV) in Shigella. Fluorescent alerts generated by an energetic GFP-VirB fusion type 2, 3, or Four discrete foci within the bacterial cytoplasm, predominantly on the quarter cell place. These alerts are fully dependent upon VirB interacting with its DNA binding web site discovered both on the virulence plasmid or an engineered surrogate.
Our findings:
1) present novel perception into VirB:pINV interactions,
2) counsel that VirB might have utility as a DNA marker, and
3) elevate questions on how and why this anti-silencing protein controls virulence gene expression on pINV of Shigella spp. varieties discrete foci/hubs inside the bacterial cytoplasm.
rmbiomed
Recombinant Escherichia coli Plasmid partition protein A (parA) |
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| MBS1254579-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1065 |
Recombinant Escherichia coli Plasmid partition protein A (parA) |
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| MBS1254579-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1135 |
Recombinant Escherichia coli Plasmid partition protein A (parA) |
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| MBS1254579-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1210 |
Recombinant Escherichia coli Plasmid Partition par B protein (parB) |
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| MBS1031944-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1220 |
Recombinant Escherichia coli Plasmid Partition par B protein (parB) |
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| MBS1031944-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 875 |
Recombinant Escherichia coli Plasmid Partition par B protein (parB) |
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| MBS1031944-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1010 |
Recombinant Escherichia coli Plasmid Partition par B protein (parB) |
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| MBS1031944-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1050 |
Recombinant Escherichia coli Plasmid Partition par B protein (parB) |
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| MBS1031944-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1150 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1334981-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1334981-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1334981-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1334981-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1334981-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1207352-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1207352-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1207352-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1207352-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1207352-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1172150-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1172150-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1172150-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1172150-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1172150-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1190286-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1190286-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1190286-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1190286-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1190286-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1120526-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1120526-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1120526-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1120526-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1120526-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1052699-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1052699-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1052699-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1052699-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1052699-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1072274-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1072274-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1072274-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1072274-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1072274-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1094144-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1094144-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1094144-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1094144-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli Chromosome partition protein mukF (mukF) |
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| MBS1094144-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli Chromosome partition protein MukB (mukB), partial |
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Recombinant Escherichia coli Chromosome partition protein MukB (mukB), partial |
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| MBS1152159-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli Chromosome partition protein MukB (mukB), partial |
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| MBS1319306-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli Chromosome partition protein MukB (mukB), partial |
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| MBS1257795-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli Chromosome partition protein MukB (mukB), partial |
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| MBS1249722-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli Chromosome partition protein MukB (mukB), partial |
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| MBS1012230-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli Chromosome partition protein MukB (mukB), partial |
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| MBS1010096-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O81 Chromosome partition protein mukF (mukF) |
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| MBS1257148-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O81 Chromosome partition protein mukF (mukF) |
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| MBS1257148-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O81 Chromosome partition protein mukF (mukF) |
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| MBS1257148-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O81 Chromosome partition protein mukF (mukF) |
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| MBS1257148-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O81 Chromosome partition protein mukF (mukF) |
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| MBS1257148-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O8 Chromosome partition protein mukF (mukF) |
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| MBS1085474-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O8 Chromosome partition protein mukF (mukF) |
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| MBS1085474-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O8 Chromosome partition protein mukF (mukF) |
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| MBS1085474-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O8 Chromosome partition protein mukF (mukF) |
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| MBS1085474-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O8 Chromosome partition protein mukF (mukF) |
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| MBS1085474-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O6 Chromosome partition protein mukF (mukF) |
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| MBS1438370-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O6 Chromosome partition protein mukF (mukF) |
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| MBS1438370-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O6 Chromosome partition protein mukF (mukF) |
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| MBS1438370-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O6 Chromosome partition protein mukF (mukF) |
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| MBS1438370-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O6 Chromosome partition protein mukF (mukF) |
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| MBS1438370-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O6 Chromosome partition protein mukE (mukE) |
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| MBS1482450-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1115 |
Recombinant Escherichia coli O6 Chromosome partition protein mukE (mukE) |
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| MBS1482450-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 730 |
Recombinant Escherichia coli O6 Chromosome partition protein mukE (mukE) |
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| MBS1482450-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 895 |
Recombinant Escherichia coli O6 Chromosome partition protein mukE (mukE) |
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| MBS1482450-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 880 |
Recombinant Escherichia coli O6 Chromosome partition protein mukE (mukE) |
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| MBS1482450-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1050 |
Recombinant Escherichia coli O8 Chromosome partition protein MukB (mukB), partial |
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| MBS1033646-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O6 Chromosome partition protein MukB (mukB), partial |
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| MBS1432103-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O9:H4 Chromosome partition protein mukF (mukF) |
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| MBS1260116-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O9:H4 Chromosome partition protein mukF (mukF) |
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| MBS1260116-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O9:H4 Chromosome partition protein mukF (mukF) |
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| MBS1260116-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O9:H4 Chromosome partition protein mukF (mukF) |
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| MBS1260116-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O9:H4 Chromosome partition protein mukF (mukF) |
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| MBS1260116-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O45:K1 Chromosome partition protein mukF (mukF) |
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| MBS1014658-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O45:K1 Chromosome partition protein mukF (mukF) |
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| MBS1014658-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O45:K1 Chromosome partition protein mukF (mukF) |
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| MBS1014658-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O45:K1 Chromosome partition protein mukF (mukF) |
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| MBS1014658-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O45:K1 Chromosome partition protein mukF (mukF) |
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| MBS1014658-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O7:K1 Chromosome partition protein mukF (mukF) |
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| MBS1092908-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O7:K1 Chromosome partition protein mukF (mukF) |
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| MBS1092908-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O7:K1 Chromosome partition protein mukF (mukF) |
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| MBS1092908-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O7:K1 Chromosome partition protein mukF (mukF) |
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| MBS1092908-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O7:K1 Chromosome partition protein mukF (mukF) |
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| MBS1092908-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O81 Chromosome partition protein MukB (mukB), partial |
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| MBS1060610-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O127:H6 Chromosome partition protein mukF (mukF) |
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| MBS1215418-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O127:H6 Chromosome partition protein mukF (mukF) |
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| MBS1215418-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O127:H6 Chromosome partition protein mukF (mukF) |
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| MBS1215418-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O127:H6 Chromosome partition protein mukF (mukF) |
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| MBS1215418-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O127:H6 Chromosome partition protein mukF (mukF) |
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| MBS1215418-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1206044-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1206044-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1206044-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1206044-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1206044-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1378452-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1378452-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1378452-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1378452-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukF (mukF) |
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| MBS1378452-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukE (mukE) |
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| MBS1295254-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1115 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukE (mukE) |
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| MBS1295254-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 730 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukE (mukE) |
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| MBS1295254-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 895 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukE (mukE) |
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| MBS1295254-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 880 |
Recombinant Escherichia coli O157:H7 Chromosome partition protein mukE (mukE) |
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| MBS1295254-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1050 |
Recombinant Escherichia coli O139:H28 Chromosome partition protein mukF (mukF) |
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| MBS1030016-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O139:H28 Chromosome partition protein mukF (mukF) |
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| MBS1030016-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O139:H28 Chromosome partition protein mukF (mukF) |
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| MBS1030016-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O139:H28 Chromosome partition protein mukF (mukF) |
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| MBS1030016-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O139:H28 Chromosome partition protein mukF (mukF) |
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| MBS1030016-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O7:K1 Chromosome partition protein MukB (mukB), partial |
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| MBS1251941-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O9:H4 Chromosome partition protein MukB (mukB), partial |
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| MBS1006237-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O157:H7 Chromosome partition protein MukB (mukB), partial |
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| MBS1119867-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O157:H7 Chromosome partition protein MukB (mukB), partial |
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| MBS1372650-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O127:H6 Chromosome partition protein MukB (mukB), partial |
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| MBS1071532-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O45:K1 Chromosome partition protein MukB (mukB), partial |
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| MBS1045608-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O17:K52:H18 Chromosome partition protein mukF (mukF) |
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| MBS1242957-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O17:K52:H18 Chromosome partition protein mukF (mukF) |
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| MBS1242957-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O17:K52:H18 Chromosome partition protein mukF (mukF) |
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| MBS1242957-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O17:K52:H18 Chromosome partition protein mukF (mukF) |
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| MBS1242957-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O17:K52:H18 Chromosome partition protein mukF (mukF) |
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| MBS1242957-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O1:K1 / APEC Chromosome partition protein mukF (mukF) |
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| MBS1258179-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O1:K1 / APEC Chromosome partition protein mukF (mukF) |
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| MBS1258179-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O1:K1 / APEC Chromosome partition protein mukF (mukF) |
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| MBS1258179-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O1:K1 / APEC Chromosome partition protein mukF (mukF) |
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| MBS1258179-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O1:K1 / APEC Chromosome partition protein mukF (mukF) |
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| MBS1258179-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O6:K15:H31 Chromosome partition protein mukF (mukF) |
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| MBS1472280-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia coli O6:K15:H31 Chromosome partition protein mukF (mukF) |
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| MBS1472280-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia coli O6:K15:H31 Chromosome partition protein mukF (mukF) |
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| MBS1472280-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia coli O6:K15:H31 Chromosome partition protein mukF (mukF) |
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| MBS1472280-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia coli O6:K15:H31 Chromosome partition protein mukF (mukF) |
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| MBS1472280-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia coli O139:H28 Chromosome partition protein MukB (mukB), partial |
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| MBS1127088-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O6:K15:H31 Chromosome partition protein MukB (mukB), partial |
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| MBS1405682-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O1:K1 / APEC Chromosome partition protein MukB (mukB), partial |
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| MBS1079562-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli O17:K52:H18 Chromosome partition protein MukB (mukB), partial |
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| MBS1062780-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia fergusonii Chromosome partition protein mukF (mukF) |
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| MBS1154657-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1310 |
Recombinant Escherichia fergusonii Chromosome partition protein mukF (mukF) |
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| MBS1154657-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 995 |
Recombinant Escherichia fergusonii Chromosome partition protein mukF (mukF) |
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| MBS1154657-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Escherichia fergusonii Chromosome partition protein mukF (mukF) |
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| MBS1154657-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1160 |
Recombinant Escherichia fergusonii Chromosome partition protein mukF (mukF) |
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| MBS1154657-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1255 |
Recombinant Escherichia fergusonii Chromosome partition protein MukB (mukB), partial |
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| MBS1037428-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Escherichia coli Plasmid segregation protein parM (parM) |
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| MBS1019588-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1195 |
Recombinant Escherichia coli Plasmid segregation protein parM (parM) |
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| MBS1019588-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 850 |
Recombinant Escherichia coli Plasmid segregation protein parM (parM) |
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| MBS1019588-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 990 |
Recombinant Escherichia coli Plasmid segregation protein parM (parM) |
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| MBS1019588-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1020 |
Recombinant Escherichia coli Plasmid segregation protein parM (parM) |
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| MBS1019588-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1125 |
Recombinant Escherichia coli Stable plasmid inheritance protein (flmA), partial |
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| MBS1236820-005mgBaculovirus | MyBiosource | 0.05mg(Baculovirus) | EUR 905 |
Recombinant Escherichia coli Stable plasmid inheritance protein (flmA), partial |
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| MBS1236820-005mgMammalianCell | MyBiosource | 0.05mg(Mammalian-Cell) | EUR 1130 |
Recombinant Escherichia coli Stable plasmid inheritance protein (flmA), partial |
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| MBS1236820-05mgEColi | MyBiosource | 0.5mg(E-Coli) | EUR 905 |
Recombinant Escherichia coli Stable plasmid inheritance protein (flmA), partial |
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| MBS1236820-05mgYeast | MyBiosource | 0.5mg(Yeast) | EUR 1130 |
Recombinant Escherichia coli Stable plasmid inheritance protein (flmA), partial |
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| MBS1236820-1mgEColi | MyBiosource | 1mg(E-Coli) | EUR 1215 |
Recombinant Escherichia coli Hemolysin secretion protein D, plasmid (hlyD), partial |
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| MBS1164040-005mgBaculovirus | MyBiosource | 0.05mg(Baculovirus) | EUR 915 |
Recombinant Escherichia coli Hemolysin secretion protein D, plasmid (hlyD), partial |
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| MBS1164040-005mgMammalianCell | MyBiosource | 0.05mg(Mammalian-Cell) | EUR 1140 |
Recombinant Escherichia coli Hemolysin secretion protein D, plasmid (hlyD), partial |
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| MBS1164040-05mgEColi | MyBiosource | 0.5mg(E-Coli) | EUR 915 |
Recombinant Escherichia coli Hemolysin secretion protein D, plasmid (hlyD), partial |
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| MBS1164040-05mgYeast | MyBiosource | 0.5mg(Yeast) | EUR 1140 |
Recombinant Escherichia coli Hemolysin secretion protein D, plasmid (hlyD), partial |
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| MBS1164040-1mgEColi | MyBiosource | 1mg(E-Coli) | EUR 1235 |
Recombinant Escherichia coli Hemolysin, plasmid (hlyA), partial |
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| MBS1027898-1mgEColi | MyBiosource | 1mg(E-Coli) | EUR 1575 |
Simplified plasmid cloning with a common MCS design and bacterial in vivo meeting
Background: The power to clone DNA sequences shortly and exactly into plasmids is important for molecular biology research. The latest improvement of seamless cloning applied sciences has made important enhancements in plasmid development, however easy and dependable instruments are at all times fascinating for time- and labor-saving functions.
Outcomes: We developed and standardized a plasmid cloning protocol primarily based on a common MCS (A number of Cloning Web site) design and bacterial in vivo meeting. With this technique, the vector is linearized first by PCR (Polymerase Chain Response) or restriction digestion.
Then a small quantity (10 ~ 20 ng) of this linear vector could be blended with a PCR-amplified insert (5× molar ratio towards vector) and remodeled straight into competent E. coli cells to acquire the specified clones by means of in vivo meeting.
Since we used a 36-bp common MCS because the homologous linker, any PCR-amplified insert with ~ 15 bp appropriate termini could be cloned into the vector with excessive constancy and effectivity.
Thus, the necessity for redesigning insert-amplifying primers in keeping with varied vector sequences and the next PCR procedures was eradicated.
Conclusions: Our protocol considerably diminished hands-on time for making ready transformation reactions, had wonderful reliability, and was confirmed to be a fast and versatile plasmid cloning approach. The protocol incorporates principally mixing steps, making it an especially automation-friendly and promising software in trendy biology research.
Key phrases: Bacterial in vivo meeting; DNA meeting; Homologous sequence; Plasmid cloning; Common MCS.
A ProQ/FinO household protein concerned in plasmid copy quantity management favours health of micro organism carrying mcr-1-bearing IncI2 plasmids
The plasmid-encoded colistin resistance gene mcr-1 challenges the usage of polymyxins and poses a menace to public well being. Though IncI2-type plasmids are the commonest vector for spreading the mcr-1 gene, the mechanisms by which these plasmids adapt to host micro organism and preserve resistance genes stay unclear.
- Herein, we investigated the regulatory mechanism for controlling the health value of an IncI2 plasmid carrying mcr-1.
- A putative ProQ/FinO household protein encoded by the IncI2 plasmid, designated as PcnR (plasmid copy quantity repressor), balances the mcr-1 expression and micro organism health by repressing the plasmid copy quantity.
- It binds to the primary stem-loop construction of the repR mRNA to repress RepA expression, which differs from some other beforehand reported plasmid replication management mechanism.
- Plasmid invasion experiments revealed that pcnR is important for the persistence of the mcr-1-bearing IncI2 plasmid within the bacterial populations. Moreover, single-copy mcr-1 gene nonetheless exerted a health value to host micro organism, and negatively affected the persistence of the IncI2 plasmid in aggressive co-cultures.
- These findings show that sustaining mcr-1 plasmid at a single copy is important for its persistence, and clarify the considerably diminished prevalence of mcr-1 following the ban of colistin as a progress promoter in China.
Recombinant Bacillus thuringiensis Plasmid recombination enzyme |
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| MBS1225624-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1105 |
Recombinant Bacillus thuringiensis Plasmid recombination enzyme |
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| MBS1225624-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1170 |
Recombinant Bacillus thuringiensis Plasmid recombination enzyme |
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| MBS1225624-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1260 |
Recombinant Bacillus thuringiensis Recombination protein RecR (recR) |
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| MBS1074093-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1120 |
Recombinant Bacillus thuringiensis Recombination protein RecR (recR) |
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| MBS1074093-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 700 |
Recombinant Bacillus thuringiensis Recombination protein RecR (recR) |
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| MBS1074093-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 870 |
Recombinant Bacillus thuringiensis Recombination protein RecR (recR) |
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| MBS1074093-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 835 |
Recombinant Bacillus thuringiensis Recombination protein RecR (recR) |
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| MBS1074093-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1020 |
Recombinant Bacillus thuringiensis subsp. konkukian Recombination protein RecR (recR) |
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| MBS1318164-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1120 |
Recombinant Bacillus thuringiensis subsp. konkukian Recombination protein RecR (recR) |
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| MBS1318164-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 700 |
Recombinant Bacillus thuringiensis subsp. konkukian Recombination protein RecR (recR) |
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| MBS1318164-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 870 |
Recombinant Bacillus thuringiensis subsp. konkukian Recombination protein RecR (recR) |
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| MBS1318164-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 835 |
Recombinant Bacillus thuringiensis subsp. konkukian Recombination protein RecR (recR) |
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| MBS1318164-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1020 |
Recombinant Bacillus thuringiensis subsp. konkukian CCA-adding enzyme (cca) |
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| MBS1301729-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1270 |
Recombinant Bacillus thuringiensis subsp. konkukian CCA-adding enzyme (cca) |
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| MBS1301729-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 940 |
Recombinant Bacillus thuringiensis subsp. konkukian CCA-adding enzyme (cca) |
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| MBS1301729-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1060 |
Recombinant Bacillus thuringiensis subsp. konkukian CCA-adding enzyme (cca) |
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| MBS1301729-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1130 |
Recombinant Bacillus thuringiensis subsp. konkukian CCA-adding enzyme (cca) |
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| MBS1301729-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1210 |
Recombinant Bacillus sp. Plasmid recombination enzyme (pre) |
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| MBS1076786-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1285 |
Recombinant Bacillus sp. Plasmid recombination enzyme (pre) |
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| MBS1076786-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 965 |
Recombinant Bacillus sp. Plasmid recombination enzyme (pre) |
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| MBS1076786-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1080 |
Recombinant Bacillus sp. Plasmid recombination enzyme (pre) |
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| MBS1076786-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1125 |
Recombinant Bacillus sp. Plasmid recombination enzyme (pre) |
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| MBS1076786-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1230 |
Recombinant Bacillus thuringiensis subsp. konkukian 1,4-alpha-glucan branching enzyme GlgB (glgB), partial |
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| MBS1366459-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Bacillus thuringiensis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial |
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| MBS1110565-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Bacillus thuringiensis Enolase (eno) |
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| MBS1020314-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1300 |
Recombinant Bacillus thuringiensis Enolase (eno) |
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| MBS1020314-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 985 |
Recombinant Bacillus thuringiensis Enolase (eno) |
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| MBS1020314-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1095 |
Recombinant Bacillus thuringiensis Enolase (eno) |
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| MBS1020314-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1145 |
Recombinant Bacillus thuringiensis Enolase (eno) |
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| MBS1020314-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1245 |
Recombinant Bacillus thuringiensis Cry1F Protein |
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| cry1F-04B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis cry3B Protein |
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| cry3B-01B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis Vip3A Protein |
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| Vip3A-03B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis cry1ab Protein |
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| Cry1ab-02B | Creative BioMart | 100ug, 1mg | EUR 398.4 |
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Description: Insecticidal Proteins |
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Recombinant Bacillus thuringiensis cry1ab Protein |
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| cry1ab-02B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis Cry1Ac Protein |
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| Cry1Ac-11B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis Cry1Ia Protein |
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| Cry1Ia-10B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis cry2Ab Protein |
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| cry2Ab-05B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis GTPase obg (obg) |
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| MBS1260544-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1300 |
Recombinant Bacillus thuringiensis GTPase obg (obg) |
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| MBS1260544-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 980 |
Recombinant Bacillus thuringiensis GTPase obg (obg) |
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| MBS1260544-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1090 |
Recombinant Bacillus thuringiensis GTPase obg (obg) |
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| MBS1260544-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1145 |
Recombinant Bacillus thuringiensis GTPase obg (obg) |
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| MBS1260544-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1240 |
Recombinant Bacillus thuringiensis GTPase Der (der) |
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| MBS1246200-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1305 |
Recombinant Bacillus thuringiensis GTPase Der (der) |
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| MBS1246200-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 990 |
Recombinant Bacillus thuringiensis GTPase Der (der) |
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| MBS1246200-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1100 |
Recombinant Bacillus thuringiensis GTPase Der (der) |
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| MBS1246200-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1155 |
Recombinant Bacillus thuringiensis GTPase Der (der) |
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| MBS1246200-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1250 |
Recombinant Bacillus thuringiensis Cry1Da2 Protein |
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| Cry1Da2-13B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis Vip3Aa7 Protein |
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| Vip3Aa7-12B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis Protein hfq (hfq) |
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| MBS1239122-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1000 |
Recombinant Bacillus thuringiensis Protein hfq (hfq) |
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| MBS1239122-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 565 |
Recombinant Bacillus thuringiensis Protein hfq (hfq) |
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| MBS1239122-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 750 |
Recombinant Bacillus thuringiensis Protein hfq (hfq) |
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| MBS1239122-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 675 |
Recombinant Bacillus thuringiensis Protein hfq (hfq) |
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| MBS1239122-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 880 |
Recombinant Bacillus thuringiensis Formamidase (amiF) |
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| MBS1206503-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1210 |
Recombinant Bacillus thuringiensis Formamidase (amiF) |
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| MBS1206503-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 860 |
Recombinant Bacillus thuringiensis Formamidase (amiF) |
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| MBS1206503-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1000 |
Recombinant Bacillus thuringiensis Formamidase (amiF) |
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| MBS1206503-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1035 |
Recombinant Bacillus thuringiensis Formamidase (amiF) |
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| MBS1206503-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1135 |
Recombinant Bacillus thuringiensis Vip3Aa19 Protein |
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| Vip3Aa19-06B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis Cry1A.105 Protein |
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| Cry1A.105-09B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis Cry1A.105 Protein |
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| CRY1A105-09B | Creative BioMart | 100ug, 1mg | EUR 398.4 |
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Description: Insecticidal Proteins |
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Recombinant Bacillus thuringiensis Kynureninase (kynU) |
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| MBS1196644-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1300 |
Recombinant Bacillus thuringiensis Kynureninase (kynU) |
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| MBS1196644-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 980 |
Recombinant Bacillus thuringiensis Kynureninase (kynU) |
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| MBS1196644-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1090 |
Recombinant Bacillus thuringiensis Kynureninase (kynU) |
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| MBS1196644-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1145 |
Recombinant Bacillus thuringiensis Kynureninase (kynU) |
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| MBS1196644-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1240 |
Recombinant Bacillus thuringiensis Protein RecA (recA) |
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| MBS1085027-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1220 |
Recombinant Bacillus thuringiensis Protein RecA (recA) |
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| MBS1085027-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 875 |
Recombinant Bacillus thuringiensis Protein RecA (recA) |
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| MBS1085027-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1010 |
Recombinant Bacillus thuringiensis Protein RecA (recA) |
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| MBS1085027-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1050 |
Recombinant Bacillus thuringiensis Protein RecA (recA) |
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| MBS1085027-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1150 |
Recombinant Bacillus thuringiensis Cry1F-1 Protein |
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| Cry1F-1-07B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis Cry1F-2 Protein |
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| Cry1F-2-08B | Lifeasible | 100ug, 1mg | Ask for price |
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Description: Applications: ELISA; Biological test; Mouse stomach toxicity test |
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Recombinant Bacillus thuringiensis Peptidase T (pepT) |
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| MBS1071750-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1280 |
Recombinant Bacillus thuringiensis Peptidase T (pepT) |
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| MBS1071750-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 960 |
Recombinant Bacillus thuringiensis Peptidase T (pepT) |
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| MBS1071750-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1075 |
Recombinant Bacillus thuringiensis Peptidase T (pepT) |
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| MBS1071750-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1120 |
Recombinant Bacillus thuringiensis Peptidase T (pepT) |
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| MBS1071750-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1220 |
Recombinant Bacillus thuringiensis Vip4 (vip4), partial |
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| MBS9018970-01mgBaculovirus | MyBiosource | 0.1mg(Baculovirus) | EUR 1500 |
Recombinant Bacillus thuringiensis Vip4 (vip4), partial |
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| MBS9018970-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 780 |
Recombinant Bacillus thuringiensis Vip4 (vip4), partial |
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| MBS9018970-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 970 |
Recombinant Bacillus thuringiensis Vip4 (vip4), partial |
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| MBS9018970-1mgEColi | MyBiosource | 1mg(E-Coli) | EUR 1380 |
Recombinant Bacillus thuringiensis Vip4 (vip4), partial |
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| MBS9018970-1mgYeast | MyBiosource | 1mg(Yeast) | EUR 1910 |
Recombinant Bacillus thuringiensis Dihydroorotase (pyrC) |
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| MBS1194580-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1300 |
Recombinant Bacillus thuringiensis Dihydroorotase (pyrC) |
|||
| MBS1194580-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 980 |
Recombinant Bacillus thuringiensis Dihydroorotase (pyrC) |
|||
| MBS1194580-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1090 |
Recombinant Bacillus thuringiensis Dihydroorotase (pyrC) |
|||
| MBS1194580-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1145 |
Recombinant Bacillus thuringiensis Dihydroorotase (pyrC) |
|||
| MBS1194580-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1240 |
Recombinant Bacillus thuringiensis GMP reductase (guaC) |
|||
| MBS1136378-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1205 |
Recombinant Bacillus thuringiensis GMP reductase (guaC) |
|||
| MBS1136378-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 855 |
Recombinant Bacillus thuringiensis GMP reductase (guaC) |
|||
| MBS1136378-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 995 |
Recombinant Bacillus thuringiensis GMP reductase (guaC) |
|||
| MBS1136378-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1030 |
Recombinant Bacillus thuringiensis GMP reductase (guaC) |
|||
| MBS1136378-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1130 |
Recombinant Bacillus thuringiensis Uridine kinase (udk) |
|||
| MBS1147684-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1130 |
Recombinant Bacillus thuringiensis Uridine kinase (udk) |
|||
| MBS1147684-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 715 |
Recombinant Bacillus thuringiensis Uridine kinase (udk) |
|||
| MBS1147684-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 885 |
Recombinant Bacillus thuringiensis Uridine kinase (udk) |
|||
| MBS1147684-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 860 |
Recombinant Bacillus thuringiensis Uridine kinase (udk) |
|||
| MBS1147684-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1035 |
Recombinant Bacillus thuringiensis Trigger factor (tig) |
|||
| MBS1115189-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1295 |
Recombinant Bacillus thuringiensis Trigger factor (tig) |
|||
| MBS1115189-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 975 |
Recombinant Bacillus thuringiensis Trigger factor (tig) |
|||
| MBS1115189-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1090 |
Recombinant Bacillus thuringiensis Trigger factor (tig) |
|||
| MBS1115189-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1140 |
Recombinant Bacillus thuringiensis Trigger factor (tig) |
|||
| MBS1115189-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1240 |
Recombinant Bacillus thuringiensis LexA repressor (lexA) |
|||
| MBS1159357-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1125 |
Recombinant Bacillus thuringiensis LexA repressor (lexA) |
|||
| MBS1159357-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 710 |
Recombinant Bacillus thuringiensis LexA repressor (lexA) |
|||
| MBS1159357-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 880 |
Recombinant Bacillus thuringiensis LexA repressor (lexA) |
|||
| MBS1159357-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 850 |
Recombinant Bacillus thuringiensis LexA repressor (lexA) |
|||
| MBS1159357-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1030 |
Recombinant Bacillus thuringiensis Ribonuclease Z (rnz) |
|||
| MBS1031502-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1185 |
Recombinant Bacillus thuringiensis Ribonuclease Z (rnz) |
|||
| MBS1031502-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 830 |
Recombinant Bacillus thuringiensis Ribonuclease Z (rnz) |
|||
| MBS1031502-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 975 |
Recombinant Bacillus thuringiensis Ribonuclease Z (rnz) |
|||
| MBS1031502-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1000 |
Recombinant Bacillus thuringiensis Ribonuclease Z (rnz) |
|||
| MBS1031502-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1115 |
Recombinant Bacillus thuringiensis Lipoyl synthase (lipA) |
|||
| MBS1082923-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1180 |
Recombinant Bacillus thuringiensis Lipoyl synthase (lipA) |
|||
| MBS1082923-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 820 |
Recombinant Bacillus thuringiensis Lipoyl synthase (lipA) |
|||
| MBS1082923-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 965 |
Recombinant Bacillus thuringiensis Lipoyl synthase (lipA) |
|||
| MBS1082923-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 985 |
Recombinant Bacillus thuringiensis Lipoyl synthase (lipA) |
|||
| MBS1082923-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1130 |
Recombinant Bacillus thuringiensis Biotin synthase (bioB) |
|||
| MBS1098617-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1210 |
Recombinant Bacillus thuringiensis Biotin synthase (bioB) |
|||
| MBS1098617-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 860 |
Recombinant Bacillus thuringiensis Biotin synthase (bioB) |
|||
| MBS1098617-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1000 |
Recombinant Bacillus thuringiensis Biotin synthase (bioB) |
|||
| MBS1098617-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1035 |
Recombinant Bacillus thuringiensis Biotin synthase (bioB) |
|||
| MBS1098617-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1135 |
Recombinant Bacillus thuringiensis Glycerol kinase (glpK) |
|||
| MBS1059341-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1360 |
Recombinant Bacillus thuringiensis Glycerol kinase (glpK) |
|||
| MBS1059341-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 1065 |
Recombinant Bacillus thuringiensis Glycerol kinase (glpK) |
|||
| MBS1059341-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1120 |
Recombinant Bacillus thuringiensis Glycerol kinase (glpK) |
|||
| MBS1059341-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1240 |
Recombinant Bacillus thuringiensis Glycerol kinase (glpK) |
|||
| MBS1059341-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1315 |
Recombinant Bacillus thuringiensis Thymidine kinase (tdk) |
|||
| MBS1034596-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1115 |
Recombinant Bacillus thuringiensis Thymidine kinase (tdk) |
|||
| MBS1034596-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 695 |
Recombinant Bacillus thuringiensis Thymidine kinase (tdk) |
|||
| MBS1034596-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 870 |
Recombinant Bacillus thuringiensis Thymidine kinase (tdk) |
|||
| MBS1034596-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 830 |
Recombinant Bacillus thuringiensis Thymidine kinase (tdk) |
|||
| MBS1034596-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1015 |
Recombinant Bacillus thuringiensis Adenylate kinase (adk) |
|||
| MBS1048439-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1135 |
Recombinant Bacillus thuringiensis Adenylate kinase (adk) |
|||
| MBS1048439-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 720 |
Recombinant Bacillus thuringiensis Adenylate kinase (adk) |
|||
| MBS1048439-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 885 |
Recombinant Bacillus thuringiensis Adenylate kinase (adk) |
|||
| MBS1048439-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 865 |
Recombinant Bacillus thuringiensis Adenylate kinase (adk) |
|||
| MBS1048439-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1040 |
Recombinant Bacillus thuringiensis Beta-lactamase (bla) |
|||
| MBS1433395-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1160 |
Recombinant Bacillus thuringiensis Beta-lactamase (bla) |
|||
| MBS1433395-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 800 |
Recombinant Bacillus thuringiensis Beta-lactamase (bla) |
|||
| MBS1433395-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 950 |
Recombinant Bacillus thuringiensis Beta-lactamase (bla) |
|||
| MBS1433395-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 960 |
Recombinant Bacillus thuringiensis Beta-lactamase (bla) |
|||
| MBS1433395-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1115 |
Recombinant Bacillus thuringiensis Ribonuclease HII (rnhB) |
|||
| MBS1011145-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1140 |
Recombinant Bacillus thuringiensis Ribonuclease HII (rnhB) |
|||
| MBS1011145-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 770 |
Recombinant Bacillus thuringiensis Ribonuclease HII (rnhB) |
|||
| MBS1011145-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 925 |
Recombinant Bacillus thuringiensis Ribonuclease HII (rnhB) |
|||
| MBS1011145-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 925 |
Recombinant Bacillus thuringiensis Ribonuclease HII (rnhB) |
|||
| MBS1011145-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1085 |
Recombinant Bacillus thuringiensis Cytidylate kinase (cmk) |
|||
| MBS1090613-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1115 |
Recombinant Bacillus thuringiensis Cytidylate kinase (cmk) |
|||
| MBS1090613-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 730 |
Recombinant Bacillus thuringiensis Cytidylate kinase (cmk) |
|||
| MBS1090613-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 895 |
Recombinant Bacillus thuringiensis Cytidylate kinase (cmk) |
|||
| MBS1090613-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 880 |
Recombinant Bacillus thuringiensis Cytidylate kinase (cmk) |
|||
| MBS1090613-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1050 |
Recombinant Bacillus thuringiensis TnP I resolvase (tnpI) |
|||
| MBS1009099-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1165 |
Recombinant Bacillus thuringiensis TnP I resolvase (tnpI) |
|||
| MBS1009099-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 805 |
Recombinant Bacillus thuringiensis TnP I resolvase (tnpI) |
|||
| MBS1009099-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 955 |
Recombinant Bacillus thuringiensis TnP I resolvase (tnpI) |
|||
| MBS1009099-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 965 |
Recombinant Bacillus thuringiensis TnP I resolvase (tnpI) |
|||
| MBS1009099-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1115 |
Recombinant Bacillus thuringiensis Phosphopentomutase (deoB) |
|||
| MBS1166143-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1265 |
Recombinant Bacillus thuringiensis Phosphopentomutase (deoB) |
|||
| MBS1166143-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 935 |
Recombinant Bacillus thuringiensis Phosphopentomutase (deoB) |
|||
| MBS1166143-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1060 |
Recombinant Bacillus thuringiensis Phosphopentomutase (deoB) |
|||
| MBS1166143-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1125 |
Recombinant Bacillus thuringiensis Phosphopentomutase (deoB) |
|||
| MBS1166143-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1205 |
Recombinant Bacillus thuringiensis Arginine deiminase (arcA) |
|||
| MBS1192532-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1280 |
Recombinant Bacillus thuringiensis Arginine deiminase (arcA) |
|||
| MBS1192532-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 960 |
Recombinant Bacillus thuringiensis Arginine deiminase (arcA) |
|||
| MBS1192532-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 1075 |
Recombinant Bacillus thuringiensis Arginine deiminase (arcA) |
|||
| MBS1192532-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 1120 |
Recombinant Bacillus thuringiensis Arginine deiminase (arcA) |
|||
| MBS1192532-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1220 |
Recombinant Bacillus thuringiensis Thiazole synthase (thiG) |
|||
| MBS1170914-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1140 |
Recombinant Bacillus thuringiensis Thiazole synthase (thiG) |
|||
| MBS1170914-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 775 |
Recombinant Bacillus thuringiensis Thiazole synthase (thiG) |
|||
| MBS1170914-002mgYeast | MyBiosource | 0.02mg(Yeast) | EUR 930 |
Recombinant Bacillus thuringiensis Thiazole synthase (thiG) |
|||
| MBS1170914-01mgEColi | MyBiosource | 0.1mg(E-Coli) | EUR 925 |
Recombinant Bacillus thuringiensis Thiazole synthase (thiG) |
|||
| MBS1170914-01mgYeast | MyBiosource | 0.1mg(Yeast) | EUR 1085 |
Recombinant Bacillus thuringiensis Thymidylate kinase (tmk) |
|||
| MBS1065629-002mgBaculovirus | MyBiosource | 0.02mg(Baculovirus) | EUR 1125 |
Recombinant Bacillus thuringiensis Thymidylate kinase (tmk) |
|||
| MBS1065629-002mgEColi | MyBiosource | 0.02mg(E-Coli) | EUR 710 |
Prevalence of plasmid mediated fluoroquinolone resistance genes amongst enteric micro organism remoted from human and animal sources in Delta State, Nigeria
Plasmid mediated quinolone resistance (PMQR) is a public well being problem arising amongst different issues, from indiscriminate use of the floroquinolones (FQr) prophylactically in animal husbandry. This research examines the incidence of PMQR genes amongst enteric micro organism remoted from human and animal sources.
A complete of 720 (360 stool and 360 fish pond water/poultry litter) samples had been examined for fluoroquinolone resistant (FQr) micro organism. Proportion FQr was usually greater amongst human isolates than isolates from animals.
Proportion of PMQR amongst FQr isolates had been (1.05 and 4.32) % for E. coli from human and animal sources. For Salmonella spp., Shigella spp., Klebsiella spp. and Aeromonas spp., percentages PMQR had been 0.00 & 6.93, 0.00 & 6.38, 4.26 & 5.26 and 0.00 &3.03 for human and animal sources respectively, for the isolates.
The PMQR genes: qnrA, qnr B, qnr S and qep A had been 11, 15, 7 and 1 amongst a complete of 1018 FQr and 29 PMQR isolates respectively. The aac (6′)-Ib-cr gene was not detected on this research. Approximate Plasmid bands of PCR amplicon for qnr A, qnr B, qnr S and qep A respectively had been established.
The proportion of PMQR genes particularly amongst isolates from animal sources is of public well being concern because of the greater risk of a horizontal FQ resistance switch to people.
